In collaboration Iranian Medicinal Plants Society
Biotechnology and genetics
cloning and bioinformatic analysis of UGT gene in saffron (Crocus sativus L.) of Iran

Mohammad Javad Habibzadeh; Seyed Mahdi Ziaratnia; Ebrahim Dorani-Uliaie

Volume 11, Issue 3 , September 2023, , Pages 281-300

https://doi.org/10.22048/jsat.2023.403704.1492

Abstract
  Saffron is the most expensive spice in the world. The economic value of saffron is due to the existence of apocarotenoids in its stigma. Therefore, the isolation and characterization of genes involving in apocarotenoids metabolism is on particular importance. In this research, beacause the importance ...  Read More

Biotechnology and genetics
Investigating apocarotenoids and the structure of genes related to their synthesis in cultivated and wild saffron species using HPLC and bioinformatics tools

Mehdi Hassanlou; Mohammad Reza Azimi Moghaddam; Seyed Alireza Salamii; Ehsan Mohsenifard

Volume 11, Issue 2 , July 2023, , Pages 183-197

https://doi.org/10.22048/jsat.2023.403233.1491

Abstract
  Cultivated saffron (Crocus sativus L.) boasts remarkable commercial value due to its possessing three pivotal metabolites: crocin, picrocrocin, and safranal. The significance of obtaining these metabolites, particularly crocin, from sources other than cultivated saffron has grown substantially, primarily ...  Read More

Biotechnology and genetics
Investigating the expression of some genes related to the production of apocarotenoids in the growth stages of stigma and tepal of cultivated saffron and two wild species

Mehdi Hassanlou; Mohammad Reza Azimi Moghaddam; Seyed Alireza Salami; Ehsan Mohsenifard

Volume 11, Issue 1 , April 2023, , Pages 71-85

https://doi.org/10.22048/jsat.2023.387897.1482

Abstract
  Agricultural saffron Crocus sativus L. is a medicinal, widely used and expensive plant and has important effective substances: crocin, picrocrocin and safranal, which gives it the characteristics of color, taste and smell. The search for new sources producing the aforementioned apocarotenoids makes the ...  Read More

Biotechnology and genetics
Study flagellum and fliC of the causative bacterial agent of saffron corm rot disease Burkholderia gladioli pv. gladioli, a strategy in identification and prevention of disease spread

Mohammad Ramezani Kaporchali; Mohammad Reza Safarnejad; Abolghasem Ghasemi; Naser Farrokhi

Volume 10, Issue 3 , September 2022, , Pages 261-272

https://doi.org/10.22048/jsat.2022.342484.1461

Abstract
  Burkholderia gladioli pv. gladioli (Bgg) is the causative agent of saffron bacterial rot. Accurate identification methods will play an important role in disease control management. Flagellum is the main commodity of the bacteria that facilitates its movement. Furthermore, it is of particular importance ...  Read More

Biotechnology and genetics
The effect of gamma radiation on some growth and phytochemical indices of saffron (Crocus sativus)

Ali Mohammad Mohit-Ardakani; Seyed Ebrahim Seifati; Ali Izanloo; Zahra Karimi-bekr

Volume 10, Issue 3 , September 2022, , Pages 273-286

https://doi.org/10.22048/jsat.2022.326686.1451

Abstract
  In recent years, the use of saffron spice is increasing due to its medicinal properties and effective components. In addition to increasing crop production, increasing the quantity and quality of secondary metabolites is considered one of the most important objectives of plant breeding programs. On the ...  Read More

Biotechnology and genetics
identification of microsatellite molecular markers in saffron (.crocus sativus L) using RNA-Seq data

ali darvishian; Farhad Nazarian-Firouzabadi; Mostafa Darvishnia; Ahmad Ismaili

Volume 10, Issue 2 , June 2022, , Pages 149-161

https://doi.org/10.22048/jsat.2022.326684.1452

Abstract
  Saffron (Crocus sativus L.) is the most valuable spice in the world. Due to the lack of genomic information, saffron breeding has encountered many problems. To this end, generating and collecting genetic data using Next Generation Sequencing (NGS) techniques is crucial for saffron traditional and molecular ...  Read More

Biotechnology and genetics
Transcriptome Assembly and Identification of EST-SSR Markers in Crocus Sativus

Mohammadreza Rezaei; Hamidreza sharifi; Alireza Seifi

Volume 10, Issue 1 , April 2022, , Pages 41-49

https://doi.org/10.22048/jsat.2022.318852.1446

Abstract
  Crocus sativus is a triploide plant and propagating by vegetative propagation. Therefore, trait segregation and genetic diversity are limited in this plant. EST-SSR markers have some priority, for example co-dominant inheritance, locus specific and highly polymorphic against all other markers. Due to ...  Read More

Biotechnology and genetics
Criteria for production of standard pathogen-free saffron corms

Mohammad Zakiaghl; Soroor Khorramdel; Alireza Koocheki; Jafar Nabati; Ahmad Nezami; Amin mirshamsi kachki; Abdollah Mollafilabi; Parviz Rezvani Moghaddam; Mehdi Nassiri Mahallati

Volume 9, Issue 2 , July 2021, , Pages 121-141

https://doi.org/10.22048/jsat.2021.233278.1401

Abstract
  Sustainable development of saffron production as one of the major and strategic goals of Iranian agriculture requires a well-defined and organized plan. Over the past half century saffron cultivation has increased 34-fold in Iran, more than any other crop. But the average yield did not follow this development ...  Read More

Biotechnology and genetics
Effect of different levels of chitosan on the amounts and expression level of crocin and safranal genes in culture of saffron suspension (Crocus sativus L.)

Tofigh Taherkhani; Rasool Asghari Zakaria; Mansoor Omidi; Nasser Zare; mahboubeh taherkhani

Volume 9, Issue 1 , April 2021, , Pages 81-90

https://doi.org/10.22048/jsat.2020.247224.1409

Abstract
  Saffron (Crocus sativus) as an important medicinal and economical plant of Iran is rich in flavor, aroma and color, along with medicinal properties in addition to nutritional benefits. The effect of chitosan on Crocin and Safranal amounts as two important medicinal components and expression of their ...  Read More

Biotechnology and genetics
Identification of ISSR markers related to quantitative traits of Crocus Sativus L. ecotypes

Seid Mohammad Alavi-Siney; Jalal Saba; Seyyed Siamak Alavikia; Mohammad Reza Azimi

Volume 8, Issue 4 , December 2020, , Pages 598-608

https://doi.org/10.22048/jsat.2020.189000.1354

Abstract
  In order to investigate the relationship between quantitative traits and ISSR markers, an experiments were carried out under farm and laboratory conditions at Zanjan University. In this experiment, 20 primers of ISSR marker were used and the agronomic traits (including flower number, fresh weight of ...  Read More

Biotechnology and genetics
The effects of ultrasound, temperature, light, chitosan and plant growth regulators on callus induction in saffron (Crocus sativus L.)

Fariba Afkhami; Nasser Zare; Rasool Asghari; Mohammad Mehdizadeh; Behnam Firoozi

Volume 8, Issue 3 , October 2020, , Pages 361-375

https://doi.org/10.22048/jsat.2020.215454.1376

Abstract
  Saffron is one of the most important pharmaceutical plants in the world due to its valuable secondary metabolites. The aim of this study is to investigate the different factors on callus induction and growth in saffron corm explants. In the first experiment, saffron corms surface were sterilized and ...  Read More

Biotechnology and genetics
Identification and recombinant expression of Crocin synthesis isoform gene in Saffron stigma

Noraddin Hosseinpour Azad

Volume 7, Issue 4 , December 2019, , Pages 481-490

https://doi.org/10.22048/jsat.2019.133922.1302

Abstract
  The existence of isoform genes in plants led to the creation of the different types of isomeric metabolites like these glycosylated forms. This study was conducted to investigate the identification and heterologous expression of Saffron stigmas Glycosyl transferase coding isoform gene during the pollination ...  Read More

Biotechnology and genetics
Genetic Diversity of Alternaria alternata the Causal Agent of Saffron Rot in Southern and Razavi Khorasan Provinces Using Molecular Markers

Ghasem Najari; Khoshnood Nourollahi

Volume 7, Issue 3 , August 2019, , Pages 331-346

https://doi.org/10.22048/jsat.2018.113230.1277

Abstract
  Alternaria leaf spot caused by Alternaria alternata is one of the most important plant diseases in world. In order to determine genetic diversity, 50 samples were collected from saffron farms of different regions in Razavi Khorasan province (Kashmar, Torbatjam, Torbathaidaryeh, Mahvellat, Bardeskan) ...  Read More

Biotechnology and genetics
Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers

Mohammad Ali Behdani; Ali Izanloo

Volume 7, Issue 3 , August 2019, , Pages 347-358

https://doi.org/10.22048/jsat.2018.104167.1266

Abstract
  Saffron is the most valuable spice in the world. It is genetically a monomorphic clone. However, differences in phenotype and quality have been reported. The most important agro-economically phenotypic variation is the appearance of flowers with more than three stigmas. The main objective of this study ...  Read More

Biotechnology and genetics
Comparative performance of transcriptome assembly programs for saffron (Crocus sativus L.)

Maryam Vahedi; Seyed Alireza Salami; Majid Shokrpour; Hassan Rezadoost

Volume 7, Issue 1 , January 2019, , Pages 69-80

https://doi.org/10.22048/jsat.2017.87859.1235

Abstract
  Saffron (Crocus sativus L.) belonging to Iridaceae family as a source of apocarotenoids is one of the most valuable spices and medicinal plants in the world. Because of the large size and high complexity of saffron genome, its sequencing remains a challenge. The arrival of next-generation sequencing ...  Read More

Biotechnology and genetics
Water Deficit and the Effect of Silver Nanoparticles on Morphological Traits onSome Saffron Ecotypes (Crocus sativus L.) in South Khorasan

Sara Sabertanha; BARAT ALI FAKHERI; Nafiseh Mahdinezhad; Zohre Alizadeh

Volume 6, Issue 4 , January 2018, , Pages 473-485

https://doi.org/10.22048/jsat.2018.82080.1223

Abstract
  The present research was conducted to investigate the effect of water stress and silver nanoparticles on morphological traits of some saffron ecotypes. The experiment was done in form of split plot factorial in a randomized complete block design format in three replications at research farm of Birjand ...  Read More

Biotechnology and genetics
Effect of some plant growth regulators and different explants types on callus induction in saffron

Khadijeh Bagheri; Pejman Azadi; Mitra Gholami; Massoud Mir Masoumi

Volume 5, Issue 3 , July 2017, , Pages 231-239

https://doi.org/10.22048/jsat.2017.36767.1120

Abstract
  Saffron (Crocus sativus L.) is the world's most expensive spice. Moreover, it is important since it contains various drug metabolites. Saffron is a triploid (2n=3X=24) and sterile plant and it does not have any viable seeds. Because of the sterility, classical breeding of this plant is limited. Developing ...  Read More

Biotechnology and genetics
The structure of the protein and gene expression of PIC2, affecting flowering Flowers (Crocus sativus L.)

faezeh gharari

Volume 5, Issue 1 , April 2017, , Pages 73-90

https://doi.org/10.22048/jsat.2017.63141.1200

Abstract
  Saffron is grown for using its flower and red color stigma. This plant has many uses in the food industry, paints and medicine. Due to its high monetary worth, identification of genes involved in the flowering is very important. In order to better understand the molecular mechanisms involved in the onset ...  Read More

Biotechnology and genetics
Study of genetic variation in some Iranian saffron accessions using molecular markers of RAPD and ISSR

Majid Shokrpour; Zeinab Abedi; Siamak Kalantari; Seyed Alireza Salami

Volume 4, Issue 4 , January 2017, , Pages 257-265

https://doi.org/10.22048/jsat.2016.38672

Abstract
  Saffron (Crocus sativus L.) is one of the most valuable medicinal and spice herbs in the world. In spite of the ancient cultivation history in Iran, there are limited breeding studies on the plant due to its vegetative reproduction. In order to evaluation genetic diversity of Iranian saffron germplasm, ...  Read More

Biotechnology and genetics
The Evaluation of Genetic Diversity in Some Saffron (Crocus sativus L.) Samples of Iran Using RAPD Marker

Fereshteh Babaee; Zahra Tahmasebi; Hasan Feyzi; Arash Fazeli

Volume 4, Issue 4 , January 2017, , Pages 267-277

https://doi.org/10.22048/jsat.2016.39230

Abstract
  Iran is one of the important distribution centers of medicinal species, including plant saffron (Crocus sativus L.). The presence or absence of genetic diversity in common native and commercial cultivars of saffron of the country has always been one of the important questions for researchers of this ...  Read More

Biotechnology and genetics
Study of genetic diversity and some of quality and agronomic traits in saffron (Crocus sativus L.)

mahdi bayat; reza amirnia; mahdi tajbakhsh; bahattin tanyolac

Volume 4, Issue 3 , November 2016, , Pages 185-200

https://doi.org/10.22048/jsat.2016.13910

Abstract
  In order to study genetic diversity and some of the quality and agronomic traits in saffron, six cultivated ecotypes were collected from different regions of the Khorasan province (Mashhad, Torbat-Jam, Gonabad, Torbat-Heydarieh, Qaen and Birjand) and they were evaluated during two cropping seasons in ...  Read More

Biotechnology and genetics
The Effect of Different Hormones on Callus Induction, Regeneration and Multiplication of Saffron (Crocus Sativus L.) Corms

Abbas Safarnejad; Seyedeh Bibi Leyla Alamdari; Hadi Darroudi; Marzieh Dalir

Volume 4, Issue 2 , August 2016, , Pages 143-154

https://doi.org/10.22048/jsat.2016.17364

Abstract
  Saffron (Crocus sativus; Iridaceae) is an important economic and medicinal crop in Iran. The saffron that is an herbaceous triploid geophyte is used mainly as a source of secondary metabolites having aromatic and medicinal value. The plant develops annually from buds on the mother corm – i.e. a ...  Read More

Biotechnology and genetics
Study on Effect of Type of Explant and Hormone on Callus Induction and Regeneration in Saffron (Crocus sativus L.)

Mohsen Sajjadi; Maghsoud Pazhouhandeh

Volume 3, Issue 3 , October 2015, , Pages 195-202

https://doi.org/10.22048/jsat.2015.10390

Abstract
  Saffron (Crocus sativus L.) is one of the medicinal plants that contain active components and medicinal materials. Tissue culture of saffron can improve the quality and quantity of the saffron product, increase its export and the farmers’ income. In this study, 36 different types of hormone combinations ...  Read More

Agriculture
Effects of saffron (Crocus sativus L.) aqueous extract in the conversion of larvae to pupa and pupa to mature of fruit flies (Drosophila melanogaster M.)

Fatemeh Fani Yazdi; Naser Mahdavi Shahri; Khadije Nejad Shahrokhabadi; Amin Fani Yazdi; Saeed Jahedi Pour

Volume 2, Issue 2 , September 2014, , Pages 165-169

https://doi.org/10.22048/jsat.2014.7274

Abstract
  Saffron (Crocus sativusL.), a native plant from Iran and especially of Khorasan region, has a specific place for people's diet. According to several studies about effects of saffron and because there are no specific studies on the effects of saffron aqueos extract on the development of Drosophila melanogaster ...  Read More

Processing, food industry and biochemistry
Molecular detection of some plant and non-plant frauds in commercial saffron using ITS marker

Nasrin Moshtaghi; Elham Hamidi; Abdolreza Bagheri; Ahmad Sharifi; Abbas Hemati Kakhki

Volume 1, Issue 2 , March 2014, , Pages 29-38

https://doi.org/10.22048/jsat.2014.4816

Abstract
  Saffron (Crocus sativus L.) is the most valuable food additive in the world which little production and high price of it caused some adulterations such as plant and chemical material similar to saffron. There are several methods for detecting these fraudulent based on morphological and chemical tests ...  Read More