Agriculture
Hamidreza sharifi; zohreh nabipour; mohammad joleini
Abstract
In order to investigate the effect of different methods and levels of irrigation water on some vegetative and reproductive characteristics of saffron, this research was carried out in the form of a split-plot experiment in the form of a randomized complete block design with three replications at the ...
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In order to investigate the effect of different methods and levels of irrigation water on some vegetative and reproductive characteristics of saffron, this research was carried out in the form of a split-plot experiment in the form of a randomized complete block design with three replications at the Razavi Khorasan Agricultural and Natural Resources Research and Training Center. (Gonabad Research Station) was carried out in the crop years of 2017-2018. In this research, irrigation method treatments (including basin, drip and sprinkler (Pflex) methods) were in the main plot and different levels of irrigation water (including supplying 100, 75 and 50% of the water requirement) were in the sub plot. Results The comparison of the averages showed that in both years of the experiment, changing the irrigation method from basin to drip (tape) and sprinkler (P-flex) led to an increase in the studied properties. Reducing the amount of irrigation water from 100% to 50% of the water requirement also caused a decrease in leaf characteristics and stigma yield, but the efficiency of irrigation water consumption increased. Based on this, reducing the water requirement of saffron from 100 to 50% in the first and second year caused a 33% and 30% decrease in stigma yield, and a 32% and 37% increase in water consumption efficiency, respectively. Examining the interaction effects table showed that in the first year of the experiment, reducing the amount of irrigation from 100 to 50% caused a greater reduction in the total length of leaves per square meter under drip irrigation (tape) than the other two irrigation methods (82% in the drip method (tape) in contrast to 79% in the sprinkler (P-flex) method and 69% in the basin method). Based on this, although the positive effect and relative advantage of modern irrigation systems is decreasing with the increase in the age of the farm, it seems that it is possible to achieve optimal performance in the region by using rain irrigation sprinkler (P-flex) method in 100% of the water requirement.
Other subject about saffron
Hamidreza sharifi; zohreh nabipour; Hamidreza Tavakoli-kakhki
Abstract
In order to investigate the effect of planting density, corm weight and planting depth on the quantity and quality of corms obtained from saffron corm production fields, an experiment in the form of a factorial split plot in the form of a randomized complete block design with three replications at the ...
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In order to investigate the effect of planting density, corm weight and planting depth on the quantity and quality of corms obtained from saffron corm production fields, an experiment in the form of a factorial split plot in the form of a randomized complete block design with three replications at the Agricultural Research and Training Center and natural resources of Razavi Khorasan (Gonabad research station with latitude 34 degrees 21 minutes north and longitude 58 degrees 41 minutes east) was carried out in the crop years of 2017-2018 and 2018-2019. In this research, planting density treatment (at four levels of 60, 90, 120 and 150 corms per square meter) in the main plot and factorial planting depth (at two levels of 15 ± 2 and 25 ± 2 cm from the soil surface) and corm weight (at two levels) level of 4±1 and 8±1 g) were located in the sub-plot. The results showed that higher density increased the number of daughter corms per square meter and the total weight of daughter corms per square meter and decreased the average fresh weight of a single corm, the ratio of the total weight of harvested corms to the planted corms and the coefficient of corm multiplication. In addition to this, the increase in the weight of mother corms and surface planting caused an increase in the number of daughter corms, the average fresh weight of a single corm, the total fresh weight of all corms, and the multiplication factor. The double interaction effect of density × corm weight on the number of daughter corms, the total fresh weight of all daughter corms, the interaction effect of density × planting depth on the average weight of a single corm and the ratio of harvested to planted corm weight and the interaction effect of corm weight × planting depth On the number of daughter corms in the second year, the average fresh weight of a single corm and the total fresh weight of all the corms per square meter were significant only in the first year. Based on this, the effect of increasing the weight of mother corms on improving the fresh weight of daughter corms at a depth of 15 cm was more than that of 25 cm. In general, it seems that at least in the fields that are cultivated with the aim of obtaining seed pods in the first years, using a density of 120 pods per square meter using standard mother pods (that is, the average pod weight) 8 grams) and surface planting (i.e. planting depth of 15 cm) is recommended, but for functional farms, larger mother corms have a relative priority only in the early years, and with the age of the field, the importance of its effect decreases. Based on this, it seems that it is possible to use a different strategy with a combination of less depth and especially more density to compensate for the effect of stem weight and achieve more performance overall.
Biotechnology and genetics
Mohammadreza Rezaei; Hamidreza sharifi; Alireza Seifi
Abstract
Crocus sativus is a triploide plant and propagating by vegetative propagation. Therefore, trait segregation and genetic diversity are limited in this plant. EST-SSR markers have some priority, for example co-dominant inheritance, locus specific and highly polymorphic against all other markers. Due to ...
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Crocus sativus is a triploide plant and propagating by vegetative propagation. Therefore, trait segregation and genetic diversity are limited in this plant. EST-SSR markers have some priority, for example co-dominant inheritance, locus specific and highly polymorphic against all other markers. Due to the availability of transcriptome data, it is possible to develop EST-SSR markers and polymorphism studies in saffron. Development of EST-SSR markers in C. sativus make it possible to study genetic diversity and molecular polymorphism in different genotypes. In order to develop EST-SSR marker for C. sativus, we downloaded public available C. sativus RNA-seq data. Quality control and preprocessing of raw reads were done using FastQC and Trimmomatic tools, respectively. We performed de novo transcriptome assembly using RNA-Bloom. CD-HIT-EST was used in order to reduce redundancy in transcriptome assembly. The assembly quality was evaluated using the BUSCO software and completeness of transcriptome assembly was 90%. After achieving to high quality transcriptome assembly of C. sativus, EST-SSRs were identified by MISA tool. The EST-SSRs primers were designed using Primer3. 35459 SSR-containing sequences were detected and primer pairs were designed for them. Ten EST-SSR primer pairs were randomly selected to amplify C. sativus DNA. Seven pairs of the primers (70%) generated clear and reproducible bands with the expected size. These EST-SSR markers can be functional and useful for C. sativus genetic studies.
Agriculture
Hamidreza sharifi; zohreh nabipour; Hamid Reza Tavakkoli Kakhki
Abstract
In order to investigate the effect of compensatory behavior of planting density, corm weight and planting depth on vegetative characteristics and yield of saffron stigma (Crocus. Sativus L.), a factorial split plot experiment with a randomized complete block design with three replications in the research ...
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In order to investigate the effect of compensatory behavior of planting density, corm weight and planting depth on vegetative characteristics and yield of saffron stigma (Crocus. Sativus L.), a factorial split plot experiment with a randomized complete block design with three replications in the research center and Agricultural education and natural resources of Khorasan Razavi (Gonabad Research Station) was conducted in the cropping years 2018-2020. In this study, planting density treatment (in four levels of 60, 90, 120 and 150 corms per square meter) in the main plot and factorial planting depth (in two levels of 15±2 and 25±2 cm from the soil surface) and corm weight (in two Levels 4± 1 and 8 ± 1 gr) were located in the subplot. The results showed that increasing the density from 60 to 150 corms per square meter caused a significant increase in flower number, flower fresh weight and stigma dry weight, number of plants, number of leaves per plant, average length of a leaf and total leaf length per square meter. Accordingly, increasing the weight of coriander from 4 to 8 gr significantly increases flower number, flower fresh weight, stigma dry weight, number of plants, number of leaves per plant, average length of a leaf, total leaf length per square meter and total Leaf length per plant in the first and second years and reduced flower number, flower fresh weight and stigma dry weight in the third year. The results also showed that increasing the planting depth from 15 to 25 cm caused a significant decrease in all studied traits of saffron. The effect of dual and triple interactions on the studied traits was mainly significant. Overall, the results showed that the use of larger mother corms has a relative priority only in the early years and the importance of its effect decreases with increasing farm age. Based on this, it seems that it is possible to have a different strateg and with a combination of less depth and especially more density to compensate for the effect of corm weight and achieved more overall performance.
Other subject about saffron
Hamid Reza Tavakkoli Kakhki; Hamidreza Sharifi; Zohreh Nabipour
Abstract
In order to investigate the effects of vegetation covering and shading on the economic yield of saffron (Crocus sativus L.) a split–plot experiment based on Randomized Complete Block Design (RCBD) with three replications was conducted at the Gonabad station for two cropping years (2018-2019, 2019- ...
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In order to investigate the effects of vegetation covering and shading on the economic yield of saffron (Crocus sativus L.) a split–plot experiment based on Randomized Complete Block Design (RCBD) with three replications was conducted at the Gonabad station for two cropping years (2018-2019, 2019- 2020). Experiments included main factor, density at four levels (90, 60, 120 and 150 corms per square meter) and sub-factors of crop residue management and shading at four levels (removal of saffron residues at the end of growing season as (control), presence of saffron residues at the end of the growing season, (control) + use of 2 (t.ha-1) barley straw and finally (control) + use of shading). Due to the nature of sub-factors, the data for the second year of growth were measured and analyzed. The results of analysis of variance showed that density and cover management had a significant effect (p≤0.01) on the vegetative characteristics of saffron (number, length and leaf area). However, the effect of density and the interaction effect of density × covering on vegetative traits had not significant effect. Also, the results of measured traits related to yield showed that the effect of corm density, covering management and the interaction of corm density × type of covering on number, flower weight and economic stigma yield were significant (p≤0.01). The highest amount of dry stigma yield and fresh weight of flowers with values of 5.16 and 411 (kg.ha-1), respectively, achieved from the treatment of 150 corms per square meter density and use of cereal straw covering and the lowest of these traits with values of 2.2 and 200 (kg.ha-1) was obtained from the density of 60 corms per square meter and control. Stepwise regression analysis for variables related to saffron economic yield showed that the number of flowers per unit area alone could explain 90.59% of the variation in saffron economic performance. Considering the positive effect of covering application on adjusting soil temperature compared to the maximum daily air temperature (at least 7%) and the simultaneous positive effect of this method of cropping management with induction of flowering stage in saffron, it seems that the use of different types of covering, especially cereal straw, is recommended to stabilize and improve saffron yield due to its low cost and easier access to covering saffron fields.