Agriculture
Seyed Mohammad Moosavipoor; Hassan Feizi; hossein sahabi; Hamid-reza Fallahi
Abstract
Recognizing the potential role of seaweed in improving saffron yield and the utilization of micronutrient elements like Ca, Mg, Fe, and Mn to enhance stigma yield, an experiment was conducted. The study investigated the impact of foliar spraying of seaweed extract and selected nutritional elements on ...
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Recognizing the potential role of seaweed in improving saffron yield and the utilization of micronutrient elements like Ca, Mg, Fe, and Mn to enhance stigma yield, an experiment was conducted. The study investigated the impact of foliar spraying of seaweed extract and selected nutritional elements on saffron's quantitative and qualitative traits of saffron.This research was conducted, as factorial based on a randomized complete block design (RCBD) with four replications during 2021-2022 in Neishabur, Iran. The first factor consisted of seaweed extract (0 and 2 L.ha-1) and the second factor consisted of foliar application of nutrients including no foliar application (as control), and the defender of some elements including iron (1.5 L.ha-1 containing Fe, N, and amino acids), zinc, boron (1 L.ha-1 containing B and amino acids), manganese (1.5 L.ha-1 containing Mn, Zn and S), magnesium and calcium (2 L.ha-1 containing Ca and N). The use of all nutrients compared to the control (no-nutrients) and the use of seaweed extract compared to no-seaweed application caused an increase in flower and stigma yields. Based on the interaction effects of the experimental factors, the highest number of flowers (68.8 No.m-2) was obtained in the combined application of manganese and seaweed extract, the highest flower yield (33.5 g.m-2) was gained with the combined application of seaweed and boron, and the highest dry stigma yield (0.48 g.m-2) was obtained from the simultaneous application of magnesium and seaweed extract, which were 63.8, 16.7 and 17.0%, more than the control treatment (no-seaweed & No-nutrients), respectively. Without nutrient application, the utilization of seaweed extract resulted in a notable rise in the Picrocrocin content of the stigma by 15.9%, Crocin by 9.5%, and Safranal by 10.6% compared to the absence of seaweed application.Combined consumption of calcium with seaweed increased the content of Picrocrocin and Crocin, while simultaneous application of other nutrients with seaweed did not improve these indices. The highest and the lowest content of Safranal (37.7 and 32.35 absorption at 330 nm) were obtained from magnesium + no-seaweed and control (no-seaweed & no-nutrients) treatments, respectively. Overall, although all of the studied elements combined with seaweed extract improved stigma yield in order to increase stigma yield, application of Mg in combination to seaweed recommended and to enhance saffron quality using seaweed suggested.
Agriculture
Nasim Rezvani; Majid Pouryousef; Afshin Tavakoli
Abstract
Saffron, a cherished native plant of Iran, holds immense value, and its optimal growth and development hinge on the precise administration of nutrients and growth regulators. However, scant information exists concerning the nutritional attributes and the impacts of external application of growth regulators ...
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Saffron, a cherished native plant of Iran, holds immense value, and its optimal growth and development hinge on the precise administration of nutrients and growth regulators. However, scant information exists concerning the nutritional attributes and the impacts of external application of growth regulators on its overall performance. This study intends to scrutinize the influence of varying concentrations of forchlorfenuron and potassium nitrate on the attributes of daughter corms as well as the ultimate saffron yield. Through this investigation, a deeper understanding of the relationship between growth regulators, nutrients, and saffron production can be attained, shedding light on the potential avenues for enhancing its cultivation and yield. This experiment was conducted as a factorial based on a randomized complete block design in the research farm of Zanjan University. According to the results, the interaction effect of forchlorfenoren 2.5 and 5 mg.liter-1 and potassium nitrate 500 mg.liter-1 decreased the number of daughter corms. Characteristics such as the weight of daughter corms of more than 7 grams per square meter, corm diameter, number of flowers per square meter, and the amount of safranal, crocin, and picrocrocin showed a significant increase due to the interaction of forchlorfenoren 5 mg.liter-1 and potassium nitrate 1000 mg.liter-1. The highest wet and dry weight of the stigma was 11.49 and 1.15 grams per square meter, respectively, with the treatment of furchlorfenoren at the level of 5 mg.liter-1. Also, the use of potassium nitrate at the level of 1000 mg.liter-1 increased the fresh and dry weight of the stigma by 10.22 and 1.01 grams per square meter, respectively. The maximum stigma length was obtained in treating furchlorfenoren 10 mg.liter-1 and potassium nitrate 250 mg.liter-1. Based on the outcomes derived from foliar application experiments, it is advisable to consider utilizing a concentration of 5 mg.liter-1 for forchlorfenuron and 1000 mg.liter-1 for potassium nitrate during the period extending from March to April. This recommendation holds under the premise that environmental conditions are conducive, encompassing factors such as favorable temperatures for foliar spraying. This practice is particularly relevant to the climatic conditions prevalent in the Zanjan region.
Biotechnology and genetics
Mehdi Hassanlou; Mohammad Reza Azimi Moghaddam; Seyed Alireza Salamii; Ehsan Mohsenifard
Abstract
Cultivated saffron (Crocus sativus L.) boasts remarkable commercial value due to its possessing three pivotal metabolites: crocin, picrocrocin, and safranal. The significance of obtaining these metabolites, particularly crocin, from sources other than cultivated saffron has grown substantially, primarily ...
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Cultivated saffron (Crocus sativus L.) boasts remarkable commercial value due to its possessing three pivotal metabolites: crocin, picrocrocin, and safranal. The significance of obtaining these metabolites, particularly crocin, from sources other than cultivated saffron has grown substantially, primarily driven by native wild saffron species in Iran. In this ongoing study, High-Performance Liquid Chromatography (HPLC) has been harnessed as a potent analytical tool for the identification of these metabolites in two wild saffron species, Khazar (C. caspius) and Ziba (C. specious), alongside the cultivated variety. Furthermore, bioinformatics tools have been employed to extract nucleotide and protein sequences, thereby facilitating the prediction of protein structures for genes integral to the biosynthesis process of these notable apocarotenoids in an in-silico manner. The research findings have showcased the presence of crocin across all analyzed samples, albeit in varying quantities. Specifically, the crocin content in the cultivated saffron, Ziba, and Khazar species accounted for 26.76%, 2.8%, and 0.74% of dry weight matter, respectively. However, the amount of picrocrocin and safranal metabolites in cultivated species was 8.4 and 0.03 percent, respectively, but there were no detectable amounts of these apocarotenoids in the studied wild species. , The existence of crocin in wild species has made hope for conducting research and searching in wild species for these effective substances and implementing breeding programs or genetic manipulation for the mentioned species.
Biotechnology and genetics
Mehdi Hassanlou; Mohammad Reza Azimi Moghaddam; Seyed Alireza Salami; Ehsan Mohsenifard
Abstract
Agricultural saffron Crocus sativus L. is a medicinal, widely used and expensive plant and has important effective substances: crocin, picrocrocin and safranal, which gives it the characteristics of color, taste and smell. The search for new sources producing the aforementioned apocarotenoids makes the ...
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Agricultural saffron Crocus sativus L. is a medicinal, widely used and expensive plant and has important effective substances: crocin, picrocrocin and safranal, which gives it the characteristics of color, taste and smell. The search for new sources producing the aforementioned apocarotenoids makes the importance of research on it more obvious day by day. Since the country of Iran has diploid wild species with characteristics close to the sterile triploid cultivated species, in the present research, two species of Caspian saffron C. caspius and Zeiba C. Speciosus, along with the cultivated species, were investigated and 6 genes active in the production of the three mentioned effective substances , CCD2, ALDH, PSY, LCY, BCH, UGT74 (and a housekeeping gene TUB as an internal control gene with constant expression in all tissues) in two flower tissues, stigma and perianth (tepal) and in five stages (time) of flowering including: The stages of bud formation (immature bud), bud ready to bloom, newly opened flower, mature flower and old flower were investigated. Using of Real Time PCR, the expression ratio (fold change) to the internal control gene (with logarithm based on 2) was investigated in different genes and different and sometimes similar expression patterns were observed with the gene expression in the cultivated species in both the stigma and stigma tissues. Based on the results obtained in this study and the increase or decrease in the expression of some genes involved in the pathways of apocarotenoids production, at the same time as the growth of the stages from the bud to the senescence stage of the flower, it is expected from the three aforementioned substances or from other apocarotenoids in the metabolom of flowers, cases have been found in the non-triploid species and create approaches for the commercial use of Iranian wild saffron species and pave the way for its commercialization.
Agriculture
Zohreh Natavan; Rooholla Moradi; Mahdi Naghizadeh; Nasibeh Pourghasemian
Abstract
In order to study the effect of different chemical and organic mulch types on various soil characteristics and qualitative and quantitative yield of saffron (Crocus sativus L.), an experiment was conducted based on randomized complete block design with three replications at Qaen region in during 2018-2019. ...
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In order to study the effect of different chemical and organic mulch types on various soil characteristics and qualitative and quantitative yield of saffron (Crocus sativus L.), an experiment was conducted based on randomized complete block design with three replications at Qaen region in during 2018-2019. The experimental treatments were application of various mulch types for weed control (no weed control, hand weeding, transparent polyethylene, black polyethylene, beeswax waste, residues of grape leaf, wheat straw, pomegranate leaf, pistachio leaf, barley straw and saffron corm dunnage). The results showed that all the treatments except black and transparent polyethylene significantly improved the potassium contents of soil compared to hand weeding treatment. Treatments of beeswax waste (0.083%), pistachio leaf (0.081%) and transparent polyethylene (0.080%) included the highest amount of soil nitrogen. The highest amount of carbon (0.838%) and soil organic matter (1.17%) was also observed in beeswax waste mulch treatment. This treatment also led to soil acidification compared to other treatments. Application of all the mulches except transparent polyethylene caused a significant increase in stigmas yield of saffron compared to non-weeding conditions. Beeswax waste and wheat straw had the highest stigma yield (4.89 and 4.32 kg ha-1), respectively; which these increased the stigma yield by 213% and 177% compared to the no weeding treatment. Black polyethylene, pomegranate leaf and grape leaf did not show a significant difference with hand weeding in terms of stigma yield of saffron. The highest amount of safranal, crocin and picrocrocin in saffron was obtained to the black polyethylene mulch treatment. In general, the results confirmed that the application of all the studied mulches with the exception of transparent polyethylene led to improving soil characteristics and saffron yield compared to no weed control treatment and are recommended to farmers.
Biotechnology and genetics
Ali Mohammad Mohit-Ardakani; Seyed Ebrahim Seifati; Ali Izanloo; Zahra Karimi-bekr
Abstract
In recent years, the use of saffron spice is increasing due to its medicinal properties and effective components. In addition to increasing crop production, increasing the quantity and quality of secondary metabolites is considered one of the most important objectives of plant breeding programs. On the ...
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In recent years, the use of saffron spice is increasing due to its medicinal properties and effective components. In addition to increasing crop production, increasing the quantity and quality of secondary metabolites is considered one of the most important objectives of plant breeding programs. On the other hand, the saffron plant has low genetic diversity due to the nature of vegetative propagation and induction of variation through mutagenesis is a suitable tool to achieve the desired diversity. Gamma radiation is one of the most widely used physical mutagens in plant breeding programs. In order to investigate the effect of gamma-ray on some growth and phytochemical indices of saffron plants, an experiment was conducted in an unbalanced complete randomized design with three treatments (Control, 15 and 18 Gy) at Yazd University. In this experiment, 15 and 18 Gy doses of gamma irradiation (Co60) were applied at Karaj Nuclear Agricultural Research Institute to induce mutations on collected saffron corms from Qaen, South Khorasan in August 2017. In this study, picrocrocin, safranal and crocin were measured in a 1% aqueous solution at 254, 330 and 440 nm wavelengths, respectively. Based on the results of this experiment, the effect of irradiation on picrocrocin and crocin was significant (P <0.001) and the observed amounts of these compounds (93.77 and 263.02) at the dose of 15 Gy were significantly higher than 18 Gy (92.34 and 262.73), while in terms of safranal content, the difference between radiation levels was not significant. Leaf area index and harvest index were also significant and showed the highest amount of these two indices at 15 Gy dose (3.06 and 0.14). In this study, although gamma radiation treatment could not significantly change the number of daughter corms between different levels of radiation and control, a significant reduction in relative chlorophyll content and an average weight of corms were observed compared to the control (without radiation). In general, the results of this study showed that in phytochemical traits as well as harvest index and leaf area, 15 Gy had a significant advantage over plants without radiation, but at higher levels of gamma radiation, probably due to degradation of genetic content and its effect on some biochemical parameters, 18 Gy plants failed to show acceptable results.
Agriculture
Alijan Salariyan; Sohrab Mahmoodi; Mohammad. Ali Behdani; hamed kaveh
Abstract
In order to investigate the effect of salinity levels, bio fertilizer and nano fertilizer of Fe on quantitative and qualitative characteristics of saffron, a factorial experiment was conducted as a combined analysis in location by a randomized complete block design with three replications. Bio fertilizer ...
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In order to investigate the effect of salinity levels, bio fertilizer and nano fertilizer of Fe on quantitative and qualitative characteristics of saffron, a factorial experiment was conducted as a combined analysis in location by a randomized complete block design with three replications. Bio fertilizer was applied at four levels of zero, 500, 1000 and 1500 kg ha-1 and nanoparticles of Fe was applied at two levels of non-consumption and application of four liters per hectare. These factors were investigated in two locations with different irrigation salinity (2.29 and 4.49 dS m-1). The results showed that there were the highest values for traits of weight of flower, number of flowers, dry weight of stigma and weight of stigma + style in irrigation conditions with a salinity of 2.29 dS m-1. In the mentioned traits, under the conditions of irrigation with salinity of 2.29 dS m-1, there was no significant difference between the application and non-application levels of nano fertilizer of Fe; However, in the conditions of irrigation with salinity of 4.49 dS m-1, the application of nano fertilizer of Fe compared to non-application, caused a significant increase of 35.5, 33, 32.8 and 34.1% of these traits, respectively. Comparison of mean for triple interaction showed that at both salinity levels of 2.29 and 4.49 dS m-1, in the absence of bio fertilizer, the application of nano fertilizer of Fe could significantly increase the amount of crocin. However, in the application of different levels of bio fertilizer, the application of nano fertilizer of Fe reduced significantly the amount of crocin. The highest amount of safranal was observed in salinity of 4.49 dS m-1 and in the treatments of application of 500 and 1500 kg per hectare of bio fertilizer and application of nano fertilizer of Fe as well as non-application of bio fertilizers and nanoparticles of Fe. Irrigation with salinity of 4.49 dS m-1 in bio fertilizer levels (except for 500 kg ha-1) caused a significant increase in picrocrocin. In generally, it seems that for achieving higher yields, irrigation with less saline water and in case of irrigation with water by more than salinity of 4.2 dS m-1, the use of nano fertilizer of Fe to moderate the effects of salinity is recommended.
Agriculture
Mansoore Kermani; Shahram Amirmoradi
Abstract
This research was carried out as factorial experiment basis on randomized block design with four replications on a three-year saffron farm in Mashhad at 2017/2018 crop year. The experimental factors were different concentrations of silica (0, 0.3, 0.5, 0.75, 1, 1.2 and 1.5 lit/ha) and the number of spraying ...
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This research was carried out as factorial experiment basis on randomized block design with four replications on a three-year saffron farm in Mashhad at 2017/2018 crop year. The experimental factors were different concentrations of silica (0, 0.3, 0.5, 0.75, 1, 1.2 and 1.5 lit/ha) and the number of spraying times (Once, twice and three times). Silica spraying (with Crapsil brand) was performed on February 6, March 1 and March 6, 2017.The results showed that the main effect of silica, the number of sprays and their interaction on all studied traits were significant. Mean comparisons of the experimental factors revealed that at the concentration of 1.2 lit/ha silica and two times of spraying, the highest number of flowers per plant(693 flowers), leaf dry weight(3.56 gr/plant), fresh weight of corm(67.25 gr/plant), stigma length(3.8 cm), fresh and dry weight of stigma(1656.5 and 14.39 kg/ha respectively),Safranal (33%) and crocin (192.75%) obtained. The highest amount of picrocrocin (66.35%) was obtained at the concentration of 1.5 lit/ha and two times of spraying. Therefore, the application of silica with the concentration of 1.2 lit/ha in the two stages of spraying was the best treatment and was able to increase the number of flowers per plant, fresh weight of corms and dry weight of stigma 26.5, 106 and 21.2 percent, respectively compared with the control treatment.
Processing, food industry and biochemistry
Jalal Ghanbari; Gholamreza Khajoei-Nejad
Abstract
Modifications in saffron quality characteristics due to storage time between samples harvested from two consecutive years have not been studied well. For this purpose, saffron samples harvested from 2016 and 2017 were analyzed using proton transfer reaction-time of flight-mass spectrometry (PTR-TOF-MS) ...
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Modifications in saffron quality characteristics due to storage time between samples harvested from two consecutive years have not been studied well. For this purpose, saffron samples harvested from 2016 and 2017 were analyzed using proton transfer reaction-time of flight-mass spectrometry (PTR-TOF-MS) and ISO 3632 trade standard procedures to determine volatile compounds as well as crocin and picrocrocin contents, respectively. The results of volatile organic compounds (VOCs) analysis revealed that 11 main compounds including safranal, acetic acid, 2(5H)-furanone, isobutanal, biogenic aldehyde fragment, 4-ketoisophorone, acetaldehyde, butyrolactone, acetone/propanal, methanol, and isophorone were responsible for the overall VOCs profile of saffron, as accounted for more than 80% of the identified VOCs. Comparisons between samples stored from two years showed that safranal, acetic acid, 2(5H)-furanone, biogenic aldehyde fragment, and butyrolactone compounds were significantly increased for samples stored from 2016 (between 56-82 percent) compared with the samples obtained from 2017. On the contrary, isophorone isomers contents as safranal precursors, decreased their contents relatively. Similarly, crocin and picrocrocin contents measured by ISO method differed significantly between samples of two studied years. For all the studied samples, color and flavor factors of saffron reduced by 20 and 14% on the average for samples stored from 2016 in comparison with amples obtained from 2017, respectively. According to results of principal component analysis, change in crocin, picrocrocin, and main volatile compounds of saffron including safranal, acetic acid, 2(5H)-furanone, isobutanal, and isophorone could be considered as an indicator for distinguishing the current year’s harvested samples from the samples stored since previous years.
Biotechnology and genetics
Tofigh Taherkhani; Rasool Asghari Zakaria; Mansoor Omidi; Nasser Zare; mahboubeh taherkhani
Abstract
Saffron (Crocus sativus) as an important medicinal and economical plant of Iran is rich in flavor, aroma and color, along with medicinal properties in addition to nutritional benefits. The effect of chitosan on Crocin and Safranal amounts as two important medicinal components and expression of their ...
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Saffron (Crocus sativus) as an important medicinal and economical plant of Iran is rich in flavor, aroma and color, along with medicinal properties in addition to nutritional benefits. The effect of chitosan on Crocin and Safranal amounts as two important medicinal components and expression of their controlling genes in suspension culture of saffron was subjected as the aim of this study. For this purpose, saffron bulbs were cultured in ½ MS medium being treated with 100 and 150 mg/l of chitosan under cell suspension medium and callus optimal growth conditions. Samples were taken at 24 and 72 hours after the application of treatment in 3 replications. Measurement of secondary metabolites was done with HPLC and analysis of genes’ expression was performed with real-time PCR. The results showed that after the use of 100 and 150 mg/l of chitosan and after 24 and 72 hours, the two CsLYC and CsGT-2 genes expression significantly increased. Also, the results showed that Safranal and Crocin levels by the use of chitosan are significantly different at both harvesting times, so that 150 mg/l at harvest time of 72 hours after application of the treatment had the highest amount of Crocin and Safranal. Usage of chitosan as a bio-stimulant in the growth of medicinal and economic plants of saffron increased the amount of valuable secondary metabolites in the cell suspension culture of the plant.
Javad Tabatabaeian; Saeed Hassanian; Azam Kadkhodaie
Abstract
This research was carried out with the aim of studying the effect of zinc and potassium levels as foliar application on quantitative and qualitative traits of saffron (Crocus sativa) in Natanz city (village Badrude) in the second year of agriculture during 2019. The experiment was done as factorial layout ...
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This research was carried out with the aim of studying the effect of zinc and potassium levels as foliar application on quantitative and qualitative traits of saffron (Crocus sativa) in Natanz city (village Badrude) in the second year of agriculture during 2019. The experiment was done as factorial layout based on a randomized complete block design with three replications. Elements such as KNO3 at the levels of control (K1), 5 in thousand (K2) and 10 in thousand (K3) and Zn-EDTA at the levels of control (Z1), 5 in thousand (Z2) and 10 in thousand (Z3) were the treatments. The studied traits included quantitative traits of fresh and dry weight of the girl's corm, fresh and dry weight of mother corm, fresh and dry yield of the flower, fresh dry yield of the stigma, qualitative traits of Crocin, picocrocin and safranal. The results showed that K2 and K3 levels increased the quantitative traits, especially at K3 level. While spraying zinc only at the Z2 level quantitative traits increased and it had no significant effect on qualitative traits. The application of potassium fertilizer at the K3 level along with the Z2 had the greatest effect on increasing the quantitative traits of saffron. Perhaps one of the reasons for increasing flower yield can be attributed to the positive effects of potassium and zinc on the photosynthetic system of the plant. This increase in the amount of photosynthesis and metabolism, in turn, affects the amount of stigma production and increases dry weight of the stigma. As a result of potassium spraying, amount of crocin and picrocrocin increased from K1 to K3 by 18 and 13 %, and safranal increased from K1 to K2 and K3 increased by 30% and 48%, respectively. In the present study, there was a positive and significant correlation between the amount of Crocin, Picrocrocin and Safranal contents with quantitative characteristics in saffron. It can be said that quality traits are influenced by quantitative traits and yield components of saffron. Therefore, flowering and flower yield of saffron is also effective in improving its quality.
Agriculture
Amin Ramezani; Hossein Aroiee; Majid Azizi; Ahmad Ahmadian
Abstract
In order to find and recommend a method for improving vegetative and reproductive traits and economic yield of saffron using organic compounds of soil amendment, a split plot experiment including three irrigation intervals of 30, 50 and 70 days as main plots and three factors of vermicompost, Terracottem ...
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In order to find and recommend a method for improving vegetative and reproductive traits and economic yield of saffron using organic compounds of soil amendment, a split plot experiment including three irrigation intervals of 30, 50 and 70 days as main plots and three factors of vermicompost, Terracottem and Bolourab A, as well as control, as sub plots was carried out for three years (2014-2017) at Saffron Research Institute, Torbat-e-Heydarieh University. The results showed that the effects of treatments on all quantitative and qualitative traits, were significant (p
Processing, food industry and biochemistry
Maryam Akbarian; Fakhri Shahidi; Mohammad Javad Varidi; Arash Koocheki; sahar roshanak
Abstract
Saffron is the most expensive agricultural product in the world and Iran is the largest saffron producer in the world. Saffron contamination in different stages of the production process, in addition to quality loss leads to reducing credit in the global market and exporting. Therefore, it is necessary ...
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Saffron is the most expensive agricultural product in the world and Iran is the largest saffron producer in the world. Saffron contamination in different stages of the production process, in addition to quality loss leads to reducing credit in the global market and exporting. Therefore, it is necessary to select an appropriate method for inactivation the microbial flora of saffron. Among the common methods that used to inactivation the microorganisms, cold plasma is due to the potential benefits such as non-toxic nature, low operational costs, and a significant reduction in water consumption during decontamination, and the possibility of its use for a variety of food products has attracted much attention. Plasma is a state of ionizing gas, including ions, electrons, ultraviolet rays, and reactive species such as radicals, atoms and molecules that can ignite, which can inactivate microorganisms. in this research, cold plasma was produced using two types of gas including nitrogen and air, and the effect of plasma radiation at 0, 3, 6, 9 and 12 minutes on the chemical and microbial (Escherichia coli, Enterococcus faecalis, Mold and Yeast) properties of saffron were investigated. The results of this study showed that germicidal effect of nitrogen plasma was lower than air plasma and the plasma exposure time had a significant effect on reduction of microbial load and by increasing the time of plasma exposure, the inactivation of microorganisms increased. The maximum microbial reduction was observed in 12 minutes. Maximum reduction in microbial load was observed at 12 minutes and 18 kilovolt voltage, which reduced the population of Escherichia coli, Enterococcus faecalis, mold and yeast by 2/69, 2/48, 1/95 log cycle respectively, However, with increasing radiation time, the amount of crocin, picocrocin and safranal decreased (p˂0.05). Reduction of crocin, safranal and picocrocin in 12 minutes was 6/01, 4/04, 5/44%, respectively.
Agriculture
Ehsan Ollah Jalili; Farnaz Ganjabadi; davod habibi; alireza eivazi
Abstract
In order to increase the quality and yield of saffron, an experiment was conducted in the Alborz province, (Eshtehard), in the year 2016-2017 through the interaction of nitroxin biological fertilizer and irrigation management. The experiment was conducted as split plot in randomized complete block design ...
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In order to increase the quality and yield of saffron, an experiment was conducted in the Alborz province, (Eshtehard), in the year 2016-2017 through the interaction of nitroxin biological fertilizer and irrigation management. The experiment was conducted as split plot in randomized complete block design with 3 replications. The experimental factors included irrigation method (Surface drip Irrigation, Subsurface drip Irrigation (15 cm depth) and Irrigation is Common (conventional area) in the main plot) and biological fertilizer of nitroxin (no fertilizer (control), 2, 4 and 6 lit.ha-1 in the Subplot). The results of this study showed that simple and interaction effects of biological fertilizer application of nitroxin and irrigation management were significant in all measured traits. In order to use optimal water resources, diamond irrigation method (Subsurface drip Irrigation) can be used to reduce irrigation losses and achieve high yield in saffron production. The effects of irrigation management of Subsurface drip Irrigation diameters with biological fertilizer of nitroxin 4 lit.ha-1 were superior to other treatments, so that the highest yield of saffron stigma was observed in this treatment with 5.8 kg.ha-1.
Agriculture
Mohammad Bagher Alizadeh; Hassan Makarian; Ali Ebadi Khazine Ghadim; Ebrahim Izadi-Darbandi; Ahmad Gholami
Abstract
In recent years, in order to produce organic products and reduce environmental problems, organic and biological fertilizers are used to increase soil fertility, eliminate the nutritional needs and to improve of plants growth. In order to evaluate the effect of organic and biological fertilizers on qualitative ...
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In recent years, in order to produce organic products and reduce environmental problems, organic and biological fertilizers are used to increase soil fertility, eliminate the nutritional needs and to improve of plants growth. In order to evaluate the effect of organic and biological fertilizers on qualitative and quantitative traits of saffron, a field experiment was carried out as split plots based on a randomized complete block design with three replications at the Research Farm of Ardebil Agricultural Research Center during two growing seasons of 2015-2016 and 2016- 2017. Treatments included three levels of organic fertilizers: vermicompost (10 t.ha-1), manure (25 t.ha-1) and control in the main plots, and plant growth promoting rhizobacteria in four levels including, control, Azotobacter sp.PTCC 1658, Pseudomonas aeroginosa, Bacillus subtilis and the combination of biofertilizer (A. sp < em>. PTCC 1658+ B. subtilis+ P. aeroginosa( in sub plots. Bio fertilizers were inoculated on the saffron corms before sowing in the first year and were applied mixed with irrigation water in the second year. The results indicated that the interaction effects of organic and biological fertilizers increased the fresh weight of flowers, dry and wet yield of stigma and style and qualitative compounds including crocin (Color factor), picrocrocin (Taste factor) and safranal (Perfume factor) compared to control. So that, the application of manure and vermicompost in combination with the total of bio-fertilizers (A. sp. PTCC 1658+ B. subtilis+ P. aeroginosa), increased the dry matter of stigma from 0.86 kg.h-1 to 2.93 and 2.7 kg.h-1 respectively. By using manure fertilizer with biofertilizers, picrocrocin, safranal and crocin, were increased 73, 77 and 83 percent compared to the control respectively. Based on this experiment results, the combined use of organic and biological fertilizers in addition to reducing the environmental pollution, can enhance qualities and quantities of yield of saffron through synergistic effects.
Agriculture
Anis Rezaei; Hassan Feizi; Rohollah Moradi
Abstract
In order to evaluate the effect of last irrigation cut-off time and various fertilizer resources on quantitative and qualitative characteristics of Saffron (Crocus sativus L.) flower, an experiment was conducted in a split-plot arranged as a randomized complete block design with three replications and ...
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In order to evaluate the effect of last irrigation cut-off time and various fertilizer resources on quantitative and qualitative characteristics of Saffron (Crocus sativus L.) flower, an experiment was conducted in a split-plot arranged as a randomized complete block design with three replications and 18 treatments in a four year research farm of Jihad ministry located in Lalezar village, Bardsir region, Kerman province, in 2015-2016. The experimental treatments were irrigation cut-off time (conventional time (5 May), 15 days before the conventional time (20 April) and 15 days after conventional time (20 May)) assigned to the main plot, and nutritional resources (cow manure 40 t ha-1, hen manure15 t.ha-1, vermicompost 10 t ha-1, humic acid 2 kg ha-1, and chemical fertilizer containing 200 kg ha-1 urea and 140 kg.ha-1 P and K) as subplot. The analysis of variance results showed that the effect of irrigation cut-off treatment was significant on all the quantitative traits, but the treatment had no significant effect on qualitative traits of flower. The results showed that the delay in irrigation cut-off led to a significant decrease in other traits. As, the highest number of flowers (41.10 in m-2), fresh (145.16 kg.ha-1) and dry (19.41 kg.ha-1) weight of flower, fresh (11.12 kg.ha-1) and dry (2.02 kg.ha-1) weight of stigma were found in the earlier irrigation cut-off treatment (before the conventional time). The effect of fertilizer resources on all the studied traits was significant except for the values of picrocrocin and safranal. In all the irrigation treatments, vermicompost and cow manure significantly produced higher flower number and stigma yield than other fertilizer treatments. Dry stigma yield in chemical fertilizer (1.91 kg.ha-1), hen manure (1. 57 kg.ha-1) and humic acid (1.42 kg.ha-1) was significantly higher than control (1.26 kg.ha-1). The highest (169.7 nm) and lowest (163.1 nm) amount of crocin were obtained in control and vermicompost treatments, respectively. In general, the results showed that irrigation cut-off in April with using vermicompost fertilizer had the best conditions for saffron cultivation in the Bardsir region of Kerman.
Agriculture
Shirin Ansaryan Mahabadi; Iraj Allah dadi; Majid Ghorbani Javid; Elias Soltani
Abstract
Salicylic acid as a plant hormone plays an important role in regulating growth and physiological processes in plant. In order to study the effects of salicylic acid hormone and mother corm weight on saffron flowering properties, an experiment was conducted as factorial based on a randomized complete ...
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Salicylic acid as a plant hormone plays an important role in regulating growth and physiological processes in plant. In order to study the effects of salicylic acid hormone and mother corm weight on saffron flowering properties, an experiment was conducted as factorial based on a randomized complete block design with three replications at the saffron research field of College of Aburaihan, University of Tehran during 2016-2017. The first factor consist of salicylic acid in three levels (Normal, priming with 1 and 2 mM concentrations (and second factor was weight of corm in two level that consist of small corm (3-5 gr) and large corm (8-10 gr). The results showed that the effect of corm weight on fresh weight of flower, Length of flower, fresh weight of stigma, length of stigma, fresh weight of pistil, length of pistil and qualitative characteristics of stigma were significant. The amount of these traits was higher in terms of the use of large mother’s corms. The effect of corm weight on dry weight of stigma was not significant. Interaction between salicylic acid and corm weight on all traits was not significant but the results of mean comparisons showed that the highest amount of fresh weight of flower (99.5 kg/ha), dry weight of stigma (1.45 kg/ha) and lowest days to emergence was 47 days after planting that related to salicylic acid in 2 mM. More amount of picrocrocin as a saffron flavor was related to salicylic acid in 1 mM. Crocin percentage in the priming treatment with 2 mM salicylic acid was higher than the other two levels; amount of safranal was high at 1mM. Totally, results of this experiment indicated that planting large mother’s corms with salicylic acid led to improvement of some quantitative and qualitative characteristics of saffron stigma.
Other subject about saffron
hamed kaveh; amir salari
Abstract
In recent decades, the concept of quality of agricultural products has changed drastically and the quality of raw material is considered as a necessary prerequisite for the quality of the final processed product. With an aim of quality classification of saffron production centers in the provinces of ...
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In recent decades, the concept of quality of agricultural products has changed drastically and the quality of raw material is considered as a necessary prerequisite for the quality of the final processed product. With an aim of quality classification of saffron production centers in the provinces of Khorasan, the main components of marketability and quality of saffron (Crocin, Picrocrocin and Safranal) are determined using ultraviolet-visible light spectrum analysis and the ISO / TS 36322 standard. Product sampling was done from 14 centers of saffron production in Khorasan provinces including Safiabad, Qaen, Kashmar, Torbat Heydarieh, Khalil Abad, Bardaskan, Shahn Abad, Chakhmaq, Torbat-Jam, Faizabad, Sabzevar, Sarayan, Eshq Abad and Birjand. Harvest, stigma separation and drying was performed equally in all samples. Comparison of results to national and ISO standard showed that the Safranal content in all of the studied areas were lower than both Iran’s national standard and ISO standards. For Picrocrocin, the results for most of the studied centers were better than ISO standard. However, the results obtained from region numbers 2, 10, 11 and 12 did not reach the national standard of Iran and the products of region numbers 5, 8 and 9 were close to the lowest value in the standard. Region 12 had the lowest Crocin content which was lower than both ISO and national standards. All of the regions 2,5,10 and 14 had low Crocin content and did not reach the minimum quality of Iran’s national standard for Crocin content.
Processing, food industry and biochemistry
Elham Azarpazhooh; Ahmad Ehtiati; Parvin Sharayei
Abstract
Saffron, as the most expensive agricultural and pharmaceutical product of the world, has a special value among plants. Since the Saffron harvesting period is short, its storage for later processing requires understanding the most effective factors affecting the quality of saffron and its deterioration. ...
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Saffron, as the most expensive agricultural and pharmaceutical product of the world, has a special value among plants. Since the Saffron harvesting period is short, its storage for later processing requires understanding the most effective factors affecting the quality of saffron and its deterioration. Therefore the effects of reposition thickness, storage temperature and storage time of saffron flowers on its chemical parameters including crocin, safranal and picrocrocin values of saffron stigma and its microbial quality indicators including total count, coliform and mold contamination were modelled. This was done using multi-layer perceptron artificial neural network (ANN) and its structure and the learning parameters were optimized using genetic algorithm technique. The optimized MLP neural networkwas capable to predict the saffron quality characteristics during storage with coefficient of determinations higher than %94 and low error values (RMSE lower than 3.5 for all responses). The ANN model showed that reposition thickness has the lowest impact on chemical and microbial parameters deterioration while increasing storage temperature and time drastically increased loss of quality although the effect of storage time is lower than that of storage temperature. Overall, keeping fresh saffron flowers at a low temperature near zero degrees centigrade is necessary for maximum retention of valuable chemical compounds and minimum microbial contamination development during saffron flower storage for further processing.
Agriculture
Masoumeh Vakili-ghartavol; Saeedeh Alizadeh Salteh
Abstract
Saffron (Crocus sativus L.) is a valuable product that is moderately resistant to drought and is cultivated in environments with very different climatic conditions. The most important chemical compositions of saffron include crocin and crocetin derivatives. Saffron is used as a food colouring and flavouring ...
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Saffron (Crocus sativus L.) is a valuable product that is moderately resistant to drought and is cultivated in environments with very different climatic conditions. The most important chemical compositions of saffron include crocin and crocetin derivatives. Saffron is used as a food colouring and flavouring agent in the food industry. Moreover, it is utilized in folk medicine as antispasmodic, carminative, sedative, anti-depression and for heart disease. In this study, saffron samples from different altitudes, i.e. Marand with altitude of 1360m and Kashmar with altitude of 1000m above sea level were collected and dried in the same conditions and compared with commercial samples. Three major metabolites (crocin, picrocrocin and safranal) were quantified in both altitudes by Spectrophotometry. The results obtained showed that saffron samples from Marand had the most amounts of crocin and picrocrocin (absorbance: 306 and 118). Also, after extraction, antioxidant activity was quantified by the DPPH free radical scavenging method. The results indicated that saffron samples from Marand had higher concentration of these constituents and antioxidant activity in comparison to that of samples from Kashmar. However, due to reduction of rain and increasing drought in the Azerbaijan region, cultivation of saffron in Marand is recommended on the basis of the results of this research and high yield and quality of saffron.
Agriculture
Hassan Feizi; Abdollah Mollafilabi; Hossein Sahabi; Ahmad Ahmadian
Abstract
In order to investigate the effects of summer irrigation and conservation tillage on flower characteristics and corms behavior of Saffron (Crocus sativus L.), a field experiment was conducted during 2009- 2010, at Faculty of Agriculture, University of Torbat-e-Heydarieh, Iran. A factorial trial based ...
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In order to investigate the effects of summer irrigation and conservation tillage on flower characteristics and corms behavior of Saffron (Crocus sativus L.), a field experiment was conducted during 2009- 2010, at Faculty of Agriculture, University of Torbat-e-Heydarieh, Iran. A factorial trial based on complete randomized block design with four replications was used. The experimental treatments were all combination of summer irrigations (July, August, July + August and no irrigation) and conservation tillage (application and non- application). Based on analysis of variance, in the first and second years, summer irrigation had significant effects on number and flower yield of saffron. In the first year, the highest number of flower, flower and stigma yield of saffron significantly observed by irrigation on July + August (25 flowers per m2, 12.1 and 0.16 g.m-2, respectively,). In the second year, irrigation in August had highest significant effects on mentioned characteristics of saffron (127.4 flowers per m2, 61.4 and 0.87 g.m-2, respectively). In the first year, flower and stigma yields of saffron significantly increased by conservation tillage (by 11.3 and 11.7%), as compared to control (no conservation tillage). In addition, summer irrigation and conservation tillage significantly increased picrocrocin content.
Agriculture
Zahra Heydari; Hossein Besharati; Saeideh Maleki Farahani
Abstract
In this research, effects of chemical and biological fertilizers on qualitative and quantitative traits of saffron, were evaluated in a field experiment as randomized complete block design with three replications at Saffron Research Farm of Shahed University during growing season of 2012-2013. Experimental ...
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In this research, effects of chemical and biological fertilizers on qualitative and quantitative traits of saffron, were evaluated in a field experiment as randomized complete block design with three replications at Saffron Research Farm of Shahed University during growing season of 2012-2013. Experimental factors were chemical nitrogen fertilizer (0, 25 and 50 kg.ha-1) and plant growth promoting rhizobacteria including Pseudomonas and Bacillus as biofertilizer (inoculation and uninoculation). The results indicated that complete application of chemical fertilizer with biofertilizer enhanced yield of saffron, up to 217%, compared to control. The highest yield obtained in application of biofertilizer and 50 kg.ha-1 chemical fertilizer. It is worth noting that control (no inoculation) resulted in highest percentage of ingredients of stigma. Application of 50 kg.ha-1 of fertilizer was caused severe loss of quality traits in stigma, as safranal and crocin contents decreased by 5.13% and 10.9%, respectively. Integrated application of 25 kg.ha-1 nitrogen fertilizer and bio-fertilizer increased the picrocrocin up to 11.9% compared to control. Application of 50 kg.ha-1 nitrogen fertilizer along with biofertilizer was the efficient treatment in increment of stigma yield and qualitative characteristics of saffron leaves. The concentration of phosphorus, zinc and copper increased up to 83.05, 69.36 and 86.6%, respectively compared to control. Totally, Pseudomonas and Bacillus inoculation increased most of the qualitative and quantitative traits of saffron through nutrients uptake.
Pharmaceutical and Medical Science and Technology
B. Marjan Razavi; Mohsen Imenshahidi; Khalil Abnous; Hossein Hosseinzadeh
Abstract
(Crocus sativus L.) Commonly known as saffron, is a perennial stem less herb of the iridaceae family, widely cultivated in Iran and other countries. It is used as a flavoring and coloring agent for many thousands of years. In traditional medicine, saffron has been used for various purposes including ...
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(Crocus sativus L.) Commonly known as saffron, is a perennial stem less herb of the iridaceae family, widely cultivated in Iran and other countries. It is used as a flavoring and coloring agent for many thousands of years. In traditional medicine, saffron has been used for various purposes including abortion, as a fever reducer, an analgesic, expectorant, antispasmodic, aphrodisiac, sedative, digestive and a carminative. Various pharmacological studies have been described that saffron and its constituents exhibit different beneficial properties, including antioxidant, anticancer, anticonvulsant, antiischemic, antigenotoxic, antidote, antiapoptotic, antitussive, antidepressive, sedative and hypnotic, hypolipidemic, antinociceptive and antiinflammatory effects. Research projects have also revealed that saffron also exhibits protective effects against cardiovascular diseases including cardiac ischemia, arrhythmia, hypertension and atherosclerosis. In this review article, the effects of saffron and its active constituents on cardiovascular system were introduced.