Agriculture
Hamid-reza Fallahi; Alijan Salariyan
Abstract
This descriptive research was conducted as interviews using 125 questionnaires among the saffron farmers of Torbat-Heydariyeh, Iran, during 2020-2021. The aim of this study was to compare the most important agronomic operations related to saffron cultivation among three groups of farmers (ordinary, skilled ...
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This descriptive research was conducted as interviews using 125 questionnaires among the saffron farmers of Torbat-Heydariyeh, Iran, during 2020-2021. The aim of this study was to compare the most important agronomic operations related to saffron cultivation among three groups of farmers (ordinary, skilled and leading farmers), and to compare the methods used by the farmers with the recommendations of saffron researchers. The classification of farmers into the three mentioned groups was based on the stigma yield in relation to the field age. Before the research, the validity of the questionnaire was confirmed and its reliability was also determined using the test-retest method. The results showed that against 15% of leading farmers, none of the ordinary and skilled farmers had agricultural education. The average education of ordinary, skilled and leading farmers were 7.1, 7.5 and 10.9 years, respectively. Corm planting date of the highest number of ordinary and skilled farmers (46.2% and 35.7%, respectively) was in September, while for the leading farmers (37.5%) was in June. The average amounts of corm application by ordinary, skilled and leading farmers was 8.9, 8.9 and 10.5 ton ha-1, respectively, and the average amount recommended by researchers was 10.4 ton ha-1. All the saffron researchers recommended the corm row planting method, which is accordance with the 30, 43.1 and 44.4% procedures of ordinary, skilled and leading farmers, respectively. The minimum corm weight recommended by researchers was 8 g, which is applied by 18.7% of ordinary farmers, 25% of skilled farmers and 100% of leading farmers. Hand weeding was the most common method of weeds control among all farmers. Contrary to the recommendation of most researchers to remain saffron leaves as mulch, the leaves are mainly used as animal feed. The appropriate field age according to researcher’s recommendation was 5-6 years, while most of the farmers use the field for 7-8 years after each planting time. In general, the low level of knowledge, mass planting method (several corms in each planting point), planting of small mother corms, inappropriate planting date and long-term exploitation of the fields were the most important cases of improper field management, especially by ordinary farmers.
Agriculture
Mohammad Kafi; Hamid reza Khazaie; Hossein Abarghouei
Abstract
In order to study competition response among saffron corms, a split plot experiment was conducted on the basis of complete randomized block design with three replications in a field in Ashkezar city of Yazd province for 3 years. The main plots were irrigation with three levels (100%, 75% and 50% of ETc) ...
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In order to study competition response among saffron corms, a split plot experiment was conducted on the basis of complete randomized block design with three replications in a field in Ashkezar city of Yazd province for 3 years. The main plots were irrigation with three levels (100%, 75% and 50% of ETc) and the sub plots were fertilization management methods including control (without any fertilizer or manure), applying 10 t ha-1 cow manure, 10 t ha-1 vermicompost both by spreading, 10 t ha-1 or 5 t ha-1vermicompost buried under planting corm rows, 10 l ha-1 Humaster Saffron fertilizer for 1 or 2 times after flowering during irrigation. Results showed that under moderate water stress in comparison with control, total corm number and total corm weight per plant was declined more than 30% and 17% respectively. But average corm weight was enhanced by 12%. Total effective corm weight and total effective corm number were declined more than 50% under strict water stress in comparison with control. During 3 experimental years, total effective corm number, total effective corm weight, total corm number and total corm weight were increased by 189%, 205%, 470% and 263% respectively while average corm weight, effective corm weight ratio and effective corm number ratio were declined by 38%, 17% and 48% respectively. In summary, results showed that increasing in corm weight per plants during experimental years were mostly as a result of increasing in corm numbers. Although, big corms per small corms were decreased year by year as a result of competition among corms. Under moderate stress water, saffron plant grew up each corm weight in comparison with increasing corm number per plant while under strict water stress allocated assimilates among more corms and increases corm numbers per plant.
Agriculture
amir akbari; Alinaghi Ziaei; Seyed Mohammadreza Naghedifar; Parviz Rezvani Moghaddam; Mahdi Gholami Sharafkhane
Abstract
Saffron is one of the most valuable spices in the world and due to its high economic value and low water requirement, it is widely cultivated in eastern Iran. In this research, which took place in the research farm of the Ferdowsi University of Mashhad, Iran, in 2021-2022, the AquaCrop model to simulate ...
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Saffron is one of the most valuable spices in the world and due to its high economic value and low water requirement, it is widely cultivated in eastern Iran. In this research, which took place in the research farm of the Ferdowsi University of Mashhad, Iran, in 2021-2022, the AquaCrop model to simulate the yield of the saffron plant was calibrated and validated using field measurements. Therefore, soil moisture, biomass, and plant canopy cover area were measured with a relatively high time resolution during the growing season. Pearson's correlation coefficient, root mean square, error, index of agreement and Nash–Sutcliffe index for moisture simulation were 0.87, 7.8 mm, 0.92 and 0.62 respectively, plant biomass was 0.99, 0.3 t.ha-1, 0.99, 0.98 and also 0.98 , 5%, 0.98 and 0.93 were obtained for canopy cover . The sensitive stages of the saffron plant were determined by examining the changes in daughter corms weight, biomass, and water productivity during different stages of growth in response to water stress, and a revised scenario was proposed to improve field irrigation. By applying this scenario and running the model, the amount of daughter corms weight production increased from 5.550 to 7.693 t.ha-1 and biomass from 7.204 to 9.395 t.ha-1. The water productivity value also increased from 3.50 to 3.69 kg.m-3 and 85 mm was saved in water consumption.
Agriculture
Hesam Aryanpour; hossein sahabi; Hassan Feizi; Alijan Salariyan; Amir Salari
Abstract
Cow manure is one of the most commonly used fertilizers in saffron cultivation, but its main problem is the slow process of decomposition and the slow release of nutrients in the short term; leading to over use of chemical fertilizers by farmers. It is expected that with the introduction of nanotechnology ...
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Cow manure is one of the most commonly used fertilizers in saffron cultivation, but its main problem is the slow process of decomposition and the slow release of nutrients in the short term; leading to over use of chemical fertilizers by farmers. It is expected that with the introduction of nanotechnology in the field of agriculture, a suitable solution will be found to improve the absorption time of organic fertilizers. Cattle manure nanoparticles can reduce the decomposition time of cattle manure with higher specific surface area. Therefore, a split-plot design was carried out using two conventional methods of mulching and mixing with soil on the performance of saffron and soil characteristics to compare the performance of conventional chemical fertilizers and cow manure with nano cow manure.The main factor in seven levels (including nano cow manure in two amounts of five and 20 tons per hectare and in two forms of application of mulch and mixed with soil, normal cow manure in the amount of 20 tons per hectare and in two forms of mulch and mixed with soil and no use cow manure) and the sub-factor included NPK chemical fertilizer (at two levels of use and non-use). The results showed that by making cattle manure nanosized, its half-life in the soil decreased from about 36 weeks to 11 weeks. Nano cow manure treatment of 20 tons per hectare mixed with soil showed an increase in dry stigma yield by 43.7, 46 and 57%, respectively, compared to conventional cow manure treatments, chemical fertilizer treatments, and controls. The nano application of cow manure resulted in maintaining more moisture, less mechanical resistance in the soil, and more percentage of nutrients in the plant. In the comparison of the two methods of mulching and mixing, it was observed that in the mulching method, the amount of moisture is higher and the surface mechanical resistance is lower in the soil, and in the mixing method, the amount of nutrients in the leaves is higher. The results showed that in treatments with nano cow manure, the mixed method is preferable to mulch. By making cattle manure nano, using the mixed method instead of mulch and applying higher levels of cattle manure, was reduced the synergistic effect of chemical fertilizers in increasing yield. As in nano cow manure treatment (20 tons per hectare mixed with soil) was not observed significant yield increase due to the addition of chemical fertilizer. Also, nano cow manuew five ton per hectare treatments (mulch and mixture) showed a 22% yield increase compared to conventional cow manure treatments. It is possible to increase the yield by 22% by making cow manure nanosized, along with reducing the consumption of cow manure by 75% in the second year of saffron cultivation.
Agriculture
Zohreh Natavan; Rooholla Moradi; Mahdi Naghizadeh; Nasibeh Pourghasemian
Abstract
In order to study the effect of different chemical and organic mulch types on various soil characteristics and qualitative and quantitative yield of saffron (Crocus sativus L.), an experiment was conducted based on randomized complete block design with three replications at Qaen region in during 2018-2019. ...
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In order to study the effect of different chemical and organic mulch types on various soil characteristics and qualitative and quantitative yield of saffron (Crocus sativus L.), an experiment was conducted based on randomized complete block design with three replications at Qaen region in during 2018-2019. The experimental treatments were application of various mulch types for weed control (no weed control, hand weeding, transparent polyethylene, black polyethylene, beeswax waste, residues of grape leaf, wheat straw, pomegranate leaf, pistachio leaf, barley straw and saffron corm dunnage). The results showed that all the treatments except black and transparent polyethylene significantly improved the potassium contents of soil compared to hand weeding treatment. Treatments of beeswax waste (0.083%), pistachio leaf (0.081%) and transparent polyethylene (0.080%) included the highest amount of soil nitrogen. The highest amount of carbon (0.838%) and soil organic matter (1.17%) was also observed in beeswax waste mulch treatment. This treatment also led to soil acidification compared to other treatments. Application of all the mulches except transparent polyethylene caused a significant increase in stigmas yield of saffron compared to non-weeding conditions. Beeswax waste and wheat straw had the highest stigma yield (4.89 and 4.32 kg ha-1), respectively; which these increased the stigma yield by 213% and 177% compared to the no weeding treatment. Black polyethylene, pomegranate leaf and grape leaf did not show a significant difference with hand weeding in terms of stigma yield of saffron. The highest amount of safranal, crocin and picrocrocin in saffron was obtained to the black polyethylene mulch treatment. In general, the results confirmed that the application of all the studied mulches with the exception of transparent polyethylene led to improving soil characteristics and saffron yield compared to no weed control treatment and are recommended to farmers.
Agriculture
Hamid-Reza Fallahi; Alijan Salariyan
Abstract
This research was conducted using questionnaires and the analysis of soil samples (the content of organic matter, nitrogen, phosphorus and potassium) obtained from saffron fields of Torbat-Heydariyeh, Iran, during 2020-2021. In this research, saffron irrigation and nutrition management methods among ...
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This research was conducted using questionnaires and the analysis of soil samples (the content of organic matter, nitrogen, phosphorus and potassium) obtained from saffron fields of Torbat-Heydariyeh, Iran, during 2020-2021. In this research, saffron irrigation and nutrition management methods among three farmer groups (progressive farmers, skilled farmers and ordinary farmers) were investigated and compared with the recommendations of saffron researchers. The classification of farmers in the mentioned three classes was based on the stigma yield in relation to the field age. The results showed that most of the progressive and skilled farmers used the combined nutrition management method (organic fertilizer + chemical fertilizer with frequency of about 65%), while the single application of chemical fertilizer was the most common method among ordinary farmers (47.5%). The mean amount of organic manure application for ordinary, skilled and progressive farmers was 5.0, 6.3 and 10.7 ton ha-1year-1, respectively (less than the amount of 19.2 tons recommended by researchers) and the mean amount of nitrogen application was 49.5, 56.5 and 87 kg ha-1 (compared with 93.3 kg recommended by researchers). The application of phosphorus and particularly potassium was not a common practice among all three groups’ farmers. The average content of organic matter in the fields was 0.68%, nitrogen 0.041%, phosphorus 16.1ppm and potassium 292 ppm, which indicates the poverty of soils in terms of organic matter and to some extent nitrogen. The foliar application of nutrients for the fields of ordinary, skilled and progressive farmers was 0.23, 0.65 and 1.00 times per year, respectively, and the average irrigation times was 3.2, 3.6, and 4.6 times per year, respectively, which is less than the recommended values by experts (2.55 and 5.8 times per year, respectively). Summer irrigation (44.4% of farmers) and sprinkle irrigation method (26.8% of farmers) were more common among progressive farmers. In general, the poverty of organic matter and lack of nitrogen in the soil, low foliar application of nutrients and lack of use of modern irrigation methods were among the most important reasons for the high gap yield of saffron fields.
Agriculture
Elnaz Ghaffarzadeh; Noraddin Hosseinpour Azad; Ali Shahi; Olivia De Murtas
Abstract
Saffron with the scientific name (Crocus sativus L.) belongs to the Iridaceae family. This plant stigma is one of the most expensive spices in the world due to its rich apocarotenoids contents crocin (responsible for color), picrocrocin (bitter taste), and safranal (perfume). For quantitative analysis ...
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Saffron with the scientific name (Crocus sativus L.) belongs to the Iridaceae family. This plant stigma is one of the most expensive spices in the world due to its rich apocarotenoids contents crocin (responsible for color), picrocrocin (bitter taste), and safranal (perfume). For quantitative analysis of these metabolites by Spectroscopy and High-Performance Liquid Chromatography (HPLC UV-Vis) in Saffron stigma biphasic extract, in purpose the aim of introducing a suitable chemotype of this plant for cultivation in Ardabil province, this study was conducted in an experimental design of randomized complete blocks with three different replications. the data analysis of variance with GenStat ver. 12.1 showed that there were significant differences (P <0.01) between the studied areas based on the apocartenoids content, and the mean comparisons with the protected Fisher test showed that among the studied areas the chemotype of Onar village in Meshginshahr city Following the quantitative pattern of apocarotenoids, has a relative dominance in comparison with other regions, and on average, the amounts of crocin, picrocrocin, and safranal in the sample volume range are 0.15±0.1, 4.9±0.11 and 1±0.2 of dry matter was measured respectively. According to the obtained data, According to the obtained data, it can be inferred that this region of Ardabil province compared to other regions of this province has the necessary potential to produce this product with high quality.
Agriculture
Abbas Jalali; Faezeh Zaefarian; Benjamin Torabi; Rahmat Abbasi
Abstract
Saffron can be cultivated in many regions of the world with different climates. But the best climate for growing saffron is the Mediterranean climate with its warm and dry summers. The present study was conducted to evaluate some indicators of saffron growth in 2019-2020 in four region with different ...
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Saffron can be cultivated in many regions of the world with different climates. But the best climate for growing saffron is the Mediterranean climate with its warm and dry summers. The present study was conducted to evaluate some indicators of saffron growth in 2019-2020 in four region with different altitudes above sea level in Sari, Iran. These regions include Sari plain (level with sea level), Sarkat (350 meters above sea level), Reskat (900 meters above sea level) and Margav (1350 meters above sea level). These experiments were performed in a randomized complete block design with three replications. The treatment was includes corm size in three weight groups: small (4±2 g), medium (10±2 g) and large (15±2 g) and Measured traits include leaf area index, cumulative dry matter, growth rate, relative growth rate and saffron yield. The results showed that the trend of saffron leaf area development in all studied regions has a non-linear trend and follows a logistic-peak function. Based on the results, a significant difference was observed between the leaf area development trend among different sizes of mother corm in all regions and the trend of leaf area index changes during the growing season was higher for larger corms than for smaller corms. The trend of changes in dry matter accumulation also had a sigmoidal trend in all experiment region. Total dry matter changes were higher for larger corms. Although the trend of changes in crop growth rate in higher altitude region was lower; But the rate of growth rate fluctuation or in other words the difference between the minimum and maximum growth rate during the season in the two higher regions was less than the lower regions. The growth trend of saffron in four regions showed that it grows during the season in region with higher altitudes above sea level and following more vegetative growth and more dry matter allocation to the storage organ, larger corm is produced and the potential to increase the yield of dried saffron flowers and stigmas will increase next year.
Agriculture
Alireza Koocheki; Surur Khorramdel; fatemeh moallem banhangi
Abstract
Saffron (Crocus sativus L.) is a sterile geophyte plant that propagates vegetatively by means of a corm formation. During each growing season, saffron propagates by daughter corms produced from the mother corm. The daughter corms are formed above the mother corm. The plant can be maintained up to 8–10 ...
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Saffron (Crocus sativus L.) is a sterile geophyte plant that propagates vegetatively by means of a corm formation. During each growing season, saffron propagates by daughter corms produced from the mother corm. The daughter corms are formed above the mother corm. The plant can be maintained up to 8–10 years. In order to study the effect of corm harvesting year on daughter corm and flower yield of saffron, a four- year experiment was conducted at Research Field, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran during four growing seasons of 2015-2016, 2016-2017, 2017-2018 and 2018-2019. Treatments were four corm harvesting years that considered as one, two, three and four years aged plots. Studied traits were flower numbers per m2, fresh weight of flower per m2, dried weight of stigma per m2, number of daughter corms per m2, dried weight of daughter corms, daughter corm diameter and number and weight of daughter corms in five weight groups such as <4, 4-8, 8-12, 12-16 and >16 g. Based on the results, the highest production gains in flower numbers (67.1 flowers per m2), flower fresh weight (32.31 g.m-2) and stigma dried weight (0.761 g.m-2) were observed in the second harvesting year. Also, the maximum daughter corm number and daughter corm yield were recorded for the third harvesting year with 189.5 corms.m-2 and 603.91 g.m-2, respectively. Although the progeny corm number continued to rise each year, at the third generation corm production was dominated by corms below 12 g and this suggests a need to lift the corms and replanting at the beginning of the fourth year.
Agriculture
Basir Atarodi; Hamidreza Zabihi; Mehdi Zangiabadi
Abstract
Balanced nutrition is one of the most important methods to increase the quantity and quality of agricultural products such as saffron. Optimal application of fertilizer and impose the balance between nutrients is very important; achieving it requires a proper assessment of plant needs. Because the leaf ...
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Balanced nutrition is one of the most important methods to increase the quantity and quality of agricultural products such as saffron. Optimal application of fertilizer and impose the balance between nutrients is very important; achieving it requires a proper assessment of plant needs. Because the leaf is the main and most important tissue of plant metabolism so decomposition of its nutrients and interpretation of the results according to standard methods can provide complete information about the nutritional status of the plant, and can also be used to determine the order of nutrient requirements and fertilizer priority of the fields. In the present study, in order to investigate the nutritional status of saffron in South Khorasan province, leaf samples were collected from 23 farms and analysed for determining the macro and micronutrients concentration. Using nutrients concentration in high yielding fields, reference concentration (Cref ) was obtained for N, P and K nutrients 2.94, 0.25 and 1.22 percent respectively. As well as Fe, Mn, Zn and Cu Cref was 215.17, 27.21, 21.30 and 7.27 mg/kg respectively. After obtaining the reference concentration, the DOP value was calculated and based on that, the order of nutrient requirements was determined. The results showed that the absolute value of DOP for all nutrients studied was more than zero, in the other words, there was a nutritional imbalance in all fields. In more than 95% of cases, the nutritional imbalance was due to nutrient deficiencies, although in a few cases, the nutritional imbalance was also related to the excess of nutrients. Based on the indices of deviation from optimum percentage, among macronutrients potassium in 70% of the studied farms and among the micronutrients, manganese in 60%, and iron in 30% of the farms, had highest negative indices. Overall and based on the results of this study, it can be recommended that in saffron farms of South Khorasan province, the use of fertilizers containing potassium, iron and manganese should be given priority.
Agriculture
Surur Khorramdel; Mahmoud Mokhtari; Hoda Latifi
Abstract
The goal of meta-analysis is to provide knowledge and better understanding of the phenomena under study. The statistical technique of a large collection of analysis results from individual studies for the purpose of integrating the findings. We conducted a global meta-analysis to evaluate the effect ...
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The goal of meta-analysis is to provide knowledge and better understanding of the phenomena under study. The statistical technique of a large collection of analysis results from individual studies for the purpose of integrating the findings. We conducted a global meta-analysis to evaluate the effect of various fertilizers on saffron yield using data obtained from peer-reviewed publication (71 studies representing 14 studies on biofertilizers, 16 studies on compost, 14 studies on humic acid, 26 studies on chemical fertilizers (NPK) and 16 studies integrated application of fertilizers). Based on the results, the highest effect size was related to the impact of humic acid on dried weight of stigma (g=3.434). After humic acid, biofertilizer, chemical fertilizer and compost were computed in the next ranks. The standard error for compost (E = 0.101) was calculated less and with higher accuracy, so it is recommended with 95% confidence interval. Based on the results of Begg and Mazumdar correlation, all studies except humic acid on dried weight of stigma, humic acid on fresh weight of stigma, manure on flower number, chemical fertilizers on dried weight of stigma and chemical fertilizers on fresh weight of flower were homogeneous. Among the application of manure rate, 60 t cow manure per ha and 5 t poultry manure per ha had the highest and lowest effects, respectively. The results of meta-analysis for compost rates on dried weight of stigma indicated that 60 t per ha had the maximum impact. The highest effect of humic acid on dried weight of stigma was computed for 30 kg/ha. Based on the results it seems that there is no good long-term plan to resolve existing problems of soil fertilizers on saffron production systems and most of the experiments are based on researcher’s opinion, ease of implementation or following published articles in credible journals. Generally, the meta-analysis results provide a basis for conducting trade-off analyses to support the fertilizer management and development strategies based on available scientific findings.
Agriculture
Alijan Salariyan; Sohrab Mahmoodi; Mohammad. Ali Behdani; hamed kaveh
Abstract
In order to investigate the effect of salinity levels, bio fertilizer and nano fertilizer of Fe on quantitative and qualitative characteristics of saffron, a factorial experiment was conducted as a combined analysis in location by a randomized complete block design with three replications. Bio fertilizer ...
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In order to investigate the effect of salinity levels, bio fertilizer and nano fertilizer of Fe on quantitative and qualitative characteristics of saffron, a factorial experiment was conducted as a combined analysis in location by a randomized complete block design with three replications. Bio fertilizer was applied at four levels of zero, 500, 1000 and 1500 kg ha-1 and nanoparticles of Fe was applied at two levels of non-consumption and application of four liters per hectare. These factors were investigated in two locations with different irrigation salinity (2.29 and 4.49 dS m-1). The results showed that there were the highest values for traits of weight of flower, number of flowers, dry weight of stigma and weight of stigma + style in irrigation conditions with a salinity of 2.29 dS m-1. In the mentioned traits, under the conditions of irrigation with salinity of 2.29 dS m-1, there was no significant difference between the application and non-application levels of nano fertilizer of Fe; However, in the conditions of irrigation with salinity of 4.49 dS m-1, the application of nano fertilizer of Fe compared to non-application, caused a significant increase of 35.5, 33, 32.8 and 34.1% of these traits, respectively. Comparison of mean for triple interaction showed that at both salinity levels of 2.29 and 4.49 dS m-1, in the absence of bio fertilizer, the application of nano fertilizer of Fe could significantly increase the amount of crocin. However, in the application of different levels of bio fertilizer, the application of nano fertilizer of Fe reduced significantly the amount of crocin. The highest amount of safranal was observed in salinity of 4.49 dS m-1 and in the treatments of application of 500 and 1500 kg per hectare of bio fertilizer and application of nano fertilizer of Fe as well as non-application of bio fertilizers and nanoparticles of Fe. Irrigation with salinity of 4.49 dS m-1 in bio fertilizer levels (except for 500 kg ha-1) caused a significant increase in picrocrocin. In generally, it seems that for achieving higher yields, irrigation with less saline water and in case of irrigation with water by more than salinity of 4.2 dS m-1, the use of nano fertilizer of Fe to moderate the effects of salinity is recommended.
Agriculture
Roohollah Saeidi Aboueshaghi; Heshmat omidi; Amir bostani
Abstract
In order to investigate the effect of chicken manure and chemical fertilizers on some morphological characteristics and yield flowers and replacement corms of saffron under irrigation regimes, a split plot experiment on the basis of complete randomized block design was conducted at the shahrekord and ...
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In order to investigate the effect of chicken manure and chemical fertilizers on some morphological characteristics and yield flowers and replacement corms of saffron under irrigation regimes, a split plot experiment on the basis of complete randomized block design was conducted at the shahrekord and two consecutive crop years (2018-2019 and 2019-2020) with three replications. Irrigation as main plot at three levels (Fc (0% moisture depletion), %33 moisture depletion and %66 moisture depletion) and nutrition as sub-plot at six levels (control (without fertilizer application), 100% recommended chemical fertilizer, 100% recommended chicken manure, 25% chemical fertilizer + 75% chicken manure, 50% chemical fertilizer + 50% chicken manure and 75% chemical fertilizer + 25% chicken manure) were used. Chicken manure at a rate of 3 tons per hectare and chemical fertilizers including: urea 100 kg, potassium sulfate 150 kg, triple superphosphate 50 kg, iron sulfate 45 kg, zinc sulfate 15 kg and manganese sulfate 20 kg, all per hectare based on soil decomposition and the plant needs, were used. Also, the morphological traits and production of flowers and replacement corms was evaluated. The results showed that the interaction effect of year × irrigation × nutrition on all studied traits except leaf number, leaf length and replacement corm diameter was significant at 1% level. The mean comparison compound of the interaction of year × irrigation × nutrition showed that the highest dry yield of stigma and style in the second year, under %33 moisture depletion and 75% chicken manure + 25% chemical fertilizer treatment at the rate of 9.21 kg /ha and the lowest amount In the first year, %66 moisture depletion and control was obtained at the rate of 3.3 kg / ha. With increasing water stress intensity, the effect of 75% chicken manure + 25% chemical fertilizers treatment on the yield of replacement corm increased compared to the control and the effect of this treatment in the second year was more compared to the first year, so that in the first year and field capacity (%0 moisture depletion), increasing yield of replacement corm in this treatment compared to the control was 3.2%, in %33 moisture depletion 11.8% and in %66 moisture depletion, 26.7% , and in the second years, was respectively 11.2%, 15.5% and 38.9%. Therefore, this treatment had the highest effect on increasing the yield of saffron stigma and style in all irrigation regimes in this experiment.
Agriculture
Mansoore Kermani; Shahram Amirmoradi
Abstract
This research was carried out as factorial experiment basis on randomized block design with four replications on a three-year saffron farm in Mashhad at 2017/2018 crop year. The experimental factors were different concentrations of silica (0, 0.3, 0.5, 0.75, 1, 1.2 and 1.5 lit/ha) and the number of spraying ...
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This research was carried out as factorial experiment basis on randomized block design with four replications on a three-year saffron farm in Mashhad at 2017/2018 crop year. The experimental factors were different concentrations of silica (0, 0.3, 0.5, 0.75, 1, 1.2 and 1.5 lit/ha) and the number of spraying times (Once, twice and three times). Silica spraying (with Crapsil brand) was performed on February 6, March 1 and March 6, 2017.The results showed that the main effect of silica, the number of sprays and their interaction on all studied traits were significant. Mean comparisons of the experimental factors revealed that at the concentration of 1.2 lit/ha silica and two times of spraying, the highest number of flowers per plant(693 flowers), leaf dry weight(3.56 gr/plant), fresh weight of corm(67.25 gr/plant), stigma length(3.8 cm), fresh and dry weight of stigma(1656.5 and 14.39 kg/ha respectively),Safranal (33%) and crocin (192.75%) obtained. The highest amount of picrocrocin (66.35%) was obtained at the concentration of 1.5 lit/ha and two times of spraying. Therefore, the application of silica with the concentration of 1.2 lit/ha in the two stages of spraying was the best treatment and was able to increase the number of flowers per plant, fresh weight of corms and dry weight of stigma 26.5, 106 and 21.2 percent, respectively compared with the control treatment.
Agriculture
Elnaz Zamani; Hooman Salari; Mokhtar Ghobadi
Abstract
AbstractWe studied the effects of foliar application of iron chelate on relative leaf water content, electrolyte leakage, leaf chlorophyll content, carotenoids content, leaf soluble sugars content and proline for saffron in Kermanshah (Iran). A two-year trial (2013–2015) was conducted in the factorial ...
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AbstractWe studied the effects of foliar application of iron chelate on relative leaf water content, electrolyte leakage, leaf chlorophyll content, carotenoids content, leaf soluble sugars content and proline for saffron in Kermanshah (Iran). A two-year trial (2013–2015) was conducted in the factorial experiment based on complete randomized design (CRD) in triplicates at the research farm of Razi University; Iran. The traits were studied in the second year of experiment. The foliar iron chelated was applied at the three considered times namely early vegetative (November), mid-vegetative (January), and the end of vegetative growth (March). The seven concentrations of iron chelate were considered i.e. zero (control), 500, 1000, 1500, 2000, 2500 and 3000 ppm. The results showed that chlorophyll a, total chlorophyll and electrolyte leakage were not influenced by the time of foliar application. However, carotenoids have been affected significantly by the time (p≤0.05) and the other traits were affected very significantly (p≤0.01). The concentration of iron solution and the interaction between two factors affected all the traits highly significant (p≤0.01) apart from the electrolyte leakage which was significantly influenced (p≤0.05). The foliar application of iron chelate with the concentration of 2000 ppm leads to increase for chlorophyll a, total chlorophyll, and carotenoids in comparison with control for 45.4%, 42.4%, and 63.6% at the mid-vegetative, respectively. Soluble sugar content also has been raised for the foliar application at the end of vegetative growth for 2000 ppm for 63.5% compare with control. Also, generally can be claimed that the foliar application of iron chelate at the mid-vegetative was more effective than others.
Agriculture
Ali Zeinadini; Mir Naser Navidi; Mahnaz Eskandari; seyed alireza seyedjalali; Javad Seyed Mohammadi; Aboalhasan Moghimi; ءشاشئئشی ganjehie; alireza moghri; mohammadreza pahlavanrad
Abstract
The objective of this study was to investigate the effect of soil and land characteristics on saffron yield, their rating for preparation of a soil requirements table of this plant for land suitability studies by FAO method. First, 124 saffron farms in Khorasan Razavi, South Khorasan, Fars, Golestan, ...
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The objective of this study was to investigate the effect of soil and land characteristics on saffron yield, their rating for preparation of a soil requirements table of this plant for land suitability studies by FAO method. First, 124 saffron farms in Khorasan Razavi, South Khorasan, Fars, Golestan, Markazi and Kerman provinces were selected. In each field, suitable soil samples were taken and a land use questionnaire was completed during three years from 2017 to 2019. Soil samples were tested to determine their physicochemical properties. After preparing the data, they were statistically analyzed. Multivariate regression between yield as a dependent variable and salinity, percentage of exchangeable sodium, soil reaction, gypsum, lime, clay, sand, silt, gravel and available potassium and phosphorus of soil were studied as independent variables by stepwise method. By examining the relationships of simple regression between important and effective land characteristics and yield, land characteristics rating was performed. Then, the saffron soil requirements table prepared. The proposed table was verified with the data of 21 new farms. The results showed that available potassium, sand, soil salinity, percentage of exchangeable sodium and lime had the highest and the reaction of soil and organic carbon had the lowest range of changes. Regression results showed that the independent variables of soil salinity, ESP, lime, gypsum, gravel and available potassium and phosphorus, respectively, affect yield. Coefficient of determination of multivariate regression showed that the variables entered in the model were able to determine 95% of the variance related to the dependent variable. The largest contribution to the reduction of saffron yield is related to soil salinity, gravel, exchangeable sodium percentage and the amount of lime. Validation results showed that the yield correlation coefficient with the soil index of the proposed table for saffron is about 0.92, which indicates the acceptable accuracy of the proposed table.
Agriculture
Hamidreza sharifi; zohreh nabipour; Hamid Reza Tavakkoli Kakhki
Abstract
In order to investigate the effect of compensatory behavior of planting density, corm weight and planting depth on vegetative characteristics and yield of saffron stigma (Crocus. Sativus L.), a factorial split plot experiment with a randomized complete block design with three replications in the research ...
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In order to investigate the effect of compensatory behavior of planting density, corm weight and planting depth on vegetative characteristics and yield of saffron stigma (Crocus. Sativus L.), a factorial split plot experiment with a randomized complete block design with three replications in the research center and Agricultural education and natural resources of Khorasan Razavi (Gonabad Research Station) was conducted in the cropping years 2018-2020. In this study, planting density treatment (in four levels of 60, 90, 120 and 150 corms per square meter) in the main plot and factorial planting depth (in two levels of 15±2 and 25±2 cm from the soil surface) and corm weight (in two Levels 4± 1 and 8 ± 1 gr) were located in the subplot. The results showed that increasing the density from 60 to 150 corms per square meter caused a significant increase in flower number, flower fresh weight and stigma dry weight, number of plants, number of leaves per plant, average length of a leaf and total leaf length per square meter. Accordingly, increasing the weight of coriander from 4 to 8 gr significantly increases flower number, flower fresh weight, stigma dry weight, number of plants, number of leaves per plant, average length of a leaf, total leaf length per square meter and total Leaf length per plant in the first and second years and reduced flower number, flower fresh weight and stigma dry weight in the third year. The results also showed that increasing the planting depth from 15 to 25 cm caused a significant decrease in all studied traits of saffron. The effect of dual and triple interactions on the studied traits was mainly significant. Overall, the results showed that the use of larger mother corms has a relative priority only in the early years and the importance of its effect decreases with increasing farm age. Based on this, it seems that it is possible to have a different strateg and with a combination of less depth and especially more density to compensate for the effect of corm weight and achieved more overall performance.
Agriculture
Surur Khorramdel; Mehdi Nassiri Mahallati; Abdollah Soltan Ahmadi; Mina Hooshmand; Mohammad Javad Mostafavi
Abstract
Carbon footprint (CF) is the total amount of greenhouse gas emissions per unit of farmlands. Since the used inputs have an important role in greenhouse gas emissions, CF as an ecological indicator have been extensively applied for assessing the environmental externalities in agroecosystems. This study ...
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Carbon footprint (CF) is the total amount of greenhouse gas emissions per unit of farmlands. Since the used inputs have an important role in greenhouse gas emissions, CF as an ecological indicator have been extensively applied for assessing the environmental externalities in agroecosystems. This study was conducted to estimate the CF and carbon efficiency (CE) of saffron production systems in North Khorasan, Razavi Khorasan and South Khorasan provinces. Also, life cycle assessment analysis is calculated for quantifying the impact of saffron farming activity on the environment. Studied indices were global warming potential (GWP), acidification potential )AP) and eutrophication potential in terrestrial (UPT) and aquatic (UPA) sub-categories, N2Odirect, N2Oindirect, N2O emissions affected as volatilization and leaching, carbon inputs (Ci), carbon outputs (Co), CF and CE. The results revealed that the lowest GWP for saffron production systems was related to south Khorasan with 339.43 kg CO2 equiv./ one kg flower yield. The minimum environmental index (EcoX) was recorded for south Khorasan (0.039 EcoX/ one kg flower yield). N2O emissions in South Khorasan, Razavi Khorasan and North Khorasan provinces were estimated with 95974.51, 199674.4 and 344723 kg N2O per one ha, respectively. The largest N2O emissions affected as leaching and volatilization were calculated for North Khorasan province (with 1.21 and 24.23 kg N2O per one ha, respectively). The maximum Ci and Co were related to North Khorasan province with 117986.52 and 15135.56 kg C per one ha, respectively. The largest CF and CE were computed for North Khorasan and south Khorasan provinces with 7.8 and 0.18, respectively. It concluded that adoption on conservation and reduced tillages, N2- fixing pulses, cover crops and green manures in rotations with saffron and increased nitrogen use efficiency as ecological approaches can optimize the system performance while reducing environmental externalities and the carbon footprint of the crop cultivation. So, with relevant agro-environmental policies in saffron production systems along with the adoption of improved agronomical practices increasing flower yield with no cost the environment can be achieved effectively, efficiently and economically.
Agriculture
Mozhgan Esmaeelian; Javad Feizy; Moslem Jahani; Soodabeh Einafshar
Abstract
In the present study, the conventional solvent extraction (CSE) was compared with ultrasound-assisted extraction (UAE) and subcritical water extraction (SWE) methods for the extraction of bioactive constituents from Crocus sativus corms. The response surface methodology (RSM) based on a central composite ...
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In the present study, the conventional solvent extraction (CSE) was compared with ultrasound-assisted extraction (UAE) and subcritical water extraction (SWE) methods for the extraction of bioactive constituents from Crocus sativus corms. The response surface methodology (RSM) based on a central composite face-centred design (CCD) was used to optimize the extraction parameters. The optimum conditions of extraction were estimated to be ethanol 80%, 48˚C and 60 min in CSE, ethanol 80%, 37˚C, 45 min, and 37 kHz in the UAE, and 180˚C and 22 min in SWE. The subcritical water extraction showed the maximum extraction yield, and the highest value of total phenolic compounds (807.6 mg gallic acid equivalent in 100 g saffron corm) and total flavonoid (12.2 mg Quercetin equivalent in 100 g saffron corm) were determined in the SWE extract. The phenolic and flavonoid content of the SWE extract is 8 and 12 times higher than the other two methods, respectively. Also, CSE and SWE extracts showed the least and the most antioxidant activities, respectively. The antibacterial activity of the optimized extracts was evaluated by the microdilution method. The results showed the minimum bactericidal concentrations of 300 and 600 mg mL-1 against Staphylococcus aureus and Escherichia coli bacteria, respectively.
Agriculture
Fatemeh Tashakkori; Ali Mohammadi Torkashvand; Abbas Ahmadi; Mehrdad Esfandiari
Abstract
Saffron (Crocus sativus L.) is one of the most expensive crop which is grown in restricted areas of the world. Due to its economic values, some farmers, based on similarities of climatic conditions have cultivated it in some regions of country regardless of land capability and suitability, which sometimes ...
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Saffron (Crocus sativus L.) is one of the most expensive crop which is grown in restricted areas of the world. Due to its economic values, some farmers, based on similarities of climatic conditions have cultivated it in some regions of country regardless of land capability and suitability, which sometimes the result was not satisfactory. Saffron yield prediction based on soil properties enables us to assess the land suitably for cultivation of this valuable plant. For this purpose, 100 soil samples were collected from Vamenan Saffron fields in Golestan province and the soil chemical and physical properties including the percentage of constituents of the mineral part of soil texture (Sand, Silt, Clay), Phosphorus, potassium, Nitrogen, pH, Electrical Conductivity (EC), Organic matter and Calcium Carbonate Equivalent were measured. In addition, the weight of Saffron wet flower (kg.Ha-1) was measured. In the present study, various combinations of soil properties as input were applied and nine models were developed using artificial neural networks and multiple linear regression models for predicting the saffron yield. Performance of the models was validated using Root Mean Square Error (RMSE), Correlation Coefficient (R) and Geometric Mean of Error Ratio (GMER) methods. The results of the correlation analyses showed phosphorus and organic matter are most effective factors in the production of Saffron. Results showed that performance of the models is much different where R2 value varies from 0.45 to 0.89. Comparing the performance of Saffron yield estimation models indicated the optimal model was obtained from the combination of phosphorous, organic matter, potassium and calcium carbonate equivalent as input and values of R2 and RMSE equal to 0.874 and 0.996 kg.ha-1, respectively.Evaluation of model results indicated that the coefficient varied was obtained from 0.45 to 0.89. The best model in saffron yield estimation was obtained when phosphorous, organic matter, potassium and electrical conductivity were as the input, so that values of R2 and root mean square error (RMSE) were obtained 0.891 and 0.89 kg.ha-1, respectively.
Agriculture
Surur Khorramdel; Fatemeh Moallem Banhangi; Javad Shabahang
Abstract
Optimization is one management approach to improve yield and resource efficiencies and decrease environmental pollutions in saffron agroecosystems. Response- surface methodology (RSM) is defined as a set of statistical techniques that are used to optimize a product. In this work, optimization of cow ...
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Optimization is one management approach to improve yield and resource efficiencies and decrease environmental pollutions in saffron agroecosystems. Response- surface methodology (RSM) is defined as a set of statistical techniques that are used to optimize a product. In this work, optimization of cow manure and leaf spraying with Dalfard on saffron using RSM was done. An experiment was conducted using central composite design with 13 treatments and two replications at the Agricultural Research Field of Ferdowsi University of Mashhad during two growing seasons of 2015-2016 and 2016-2017. The treatments were allocated based on low and high levels of cow manure (0 and 100 t.ha-1, respectively) and leaf spraying concentration with Dalfard ® (0 and 10 ppm, respectively). Flower yield, daughter corm yield and quality criteria were calculated as dependent variables and changes of these variables were evaluated by a regression model. Lack-of-fit test was used to evaluate the quality of the fitted model. The adequacy of the model was tested by analysis of variance. The quality of the fitted models was judged using the determination coefficient (R2). Finally, the optimum levels of cow manure and leaf spraying concentration were calculated based on economic scenario. The results showed that effect of linear component was significant on quality characteristics of stigma. Effect of square component was significant on dried weight of stigma, mean diameter of daughter corm, number of daughter corms per m2, mean weight of daughter corm and crocin content. Interaction effect of full quadratic component was significant on flower yield criteria. Lack of fit test had no significant effect on the studied traits that represent the full square model was satisfactorily explained. The maximum observed value for number of daughter corms was recorded for 50 t cow manure per ha+ no leaf spraying (with 41.38 corms.m-2). The highest observed value for dried stigma yield was related to 100 t cow manure per ha+ no leaf spraying (with 156.33 mg.m-2). The maximum observed amounts for quality criteria such as picrocrocin, crocin and safranal were calculated for 100 t cow manure per ha+ 10 ppm leaf spraying (with 89.91 E1%257, 180.98 E1%440 and 40.15 E1%330, respectively). Dried weight of stigma, number of daughter corms and crocin content were considered in economic scenario, so the estimated levels for cow manure and Dalfard concentration were 92.67 t/ha and 1.2 ppm, respectively. In general, it seems that resource use optimization based on Response- surface methodology may be suitable cropping approach for sustainable production and improvement of yield flower, corm yield and quality characteristics in saffron.
Agriculture
Hamid-reza Fallahi; Mohammad. Ali Behdani; Parviz Rezvani Moghaddam; Majid Jami Al-Ahmadi
Abstract
Many activities during production, processing, trade and consumption of saffron (Crocus sativus L.) in Iran, are based on indigenous knowledge. Most share of saffron production in the country is obtained from small fields with family cooperation and using local environmental friendly inputs and technologies. ...
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Many activities during production, processing, trade and consumption of saffron (Crocus sativus L.) in Iran, are based on indigenous knowledge. Most share of saffron production in the country is obtained from small fields with family cooperation and using local environmental friendly inputs and technologies. Accordingly, a cosidarable part of saffron produced in Iran is close to the principles of organic farming, but in terms of rules and standards related to this production system, it is considered only as a quasi-organic product. Organic farming is a comprehensive production system that focuses on the quantitative and qualitative aspects of agricultural product during the production cycle (from farm to fork), as well as issues such as justice, social relations, soil health and the rights of all macro and mico organisms. Based on this definition and considering the principles and methods which are used for saffron production in Iranian agroecosystems, it is possible to create a targeted procrdure for its organic production, by perparation a standard. The preparation of this standard, while increasing farmers' incomes, will lead to maintaining and increasing Iran's share of the global market of this valuable medicinal plant. In this article, for all stages of saffron production cycle, using the results of scientific researches on saffron, as well as international, regional and national standards related to organic production, a set of general principles, suggestions (recommendations), requirements and notes are provided. The recommendations are mainly suitable for increasing the quantitative yield, while higher quality of saffron can be achieved by observing the proposed requirements. The guidelines proposed in this paper, besides improving the quantity and quality of the product, will also ensure the other objectives and rules related to organic farming. In the current proposed program, issues related to agronomy (corm production and selection, land selection and preparation, planting, soil nutrition, irrigation, control of biotic stresses, etc.), breeding, harvesting and post-harvest (flowers carrying and storage; separation, drying, grading, packing, labeling and storage of stigma) as well as processing and legal issues have been considered.
Agriculture
Surur Khorramdel; F. Moallem Banhangi; S.J. Davarpanah
Abstract
Saffron field from the onset of dormancy to flowering phase is free of vegetation and increased soil temperature and reduced land use efficiency are the clearest problems of sole saffron cultivation. In order to study the effect of intercropping patterns of mallow (Malva sylvetris L.) as a perennial ...
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Saffron field from the onset of dormancy to flowering phase is free of vegetation and increased soil temperature and reduced land use efficiency are the clearest problems of sole saffron cultivation. In order to study the effect of intercropping patterns of mallow (Malva sylvetris L.) as a perennial medicinal plant with saffron (Crocus sativus L.) on replacement corm growth and flower yield affected as possible cooling of corms for climate change and global warming mitigation, an experiment was conducted at Faculty of Agriculture, Ferdowsi University of Mashhad, Iran during three growing seasons of 2015-2016, 2016-2017 and 2017-2018. Treatments were 15, 30, 45 and 60-cm row spacings for saffron from mallow planting rows and sole saffron and mallow cultivations. Based on results in the third year, the effect of intercropping patterns with mallow was significant on yield indicators of flower and corm of saffron. In comparison between sole cultivation and intercropped saffron revealed that the highest values for flower number, dried stigma yield and yield of replacement corms were recorded for sole saffron cultivation with 81 flowers.m-2, 0.2115 g.m-2 and 26.51 g.m-2, respectively. In comparisons amongst intercropping patterns, the highest values for these criteria were related to 30-cm row spacings from mallow with 46 flowers.m-2, 0.155 g.m-2 and 13.39 g.m-2, respectively. However, corcin, picrocrocin and safranal contents were not significantly affected by intercropping patterns with mallow. Effect of intercropped saffron was significant on fresh weight of flower, dried weight of flower and branches number per plant of mallow. The maximum value for dried flower weight was observed for 60-cm row spacing with 28.89 g.m-2. The range for LER calculated with 1.01-1.77 and the maximum value was for 15-cm row spacing.
Agriculture
Hassan Mosaferyzyaaldiny; Amin Alizadeh; Parviz Rezvani Moghaddam
Abstract
An experiment was conducted in a strip-plot layout based on a randomized complete block design with three replications in the 2016-2017 growing season at the Bakhrez county, Khorasan Razavi province, Iran in order to study quantitative performance and water use efficiency of a few saffron criteria in ...
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An experiment was conducted in a strip-plot layout based on a randomized complete block design with three replications in the 2016-2017 growing season at the Bakhrez county, Khorasan Razavi province, Iran in order to study quantitative performance and water use efficiency of a few saffron criteria in response to different irrigation volumes and intervals. Irrigation intervals was allocated as horizontal factor (factor A) in four levels (a1=15, a2 =30, a3 = 45, and a4 = 90 days) and irrigation water volume performed as vertical factor (factor B) in four volumes (b1 = 250, b2 = 500, b3 = 1000 and b4 = 1250 m3.ha-1). The results showed that the highest saffron flower and corm yields were obtained in the a2b3 treatment (total water volume of 6000 m3.ha-1). The highest reduction in corm yield is shown in the a4b1 treatment (total water volume 750 m3.ha-1) with a decrease of 72.1% compared to a1b2 (total water volume 5500 m3.ha-1). The lowest fresh flower yield was obtained in the a4b1 treatment (total water volume 750 m3.ha-1) with a decrease of 76.5% compared to a1b2 (total water volume 5500 m3.ha-1), and the lowest yield of corm yield was shown in treatment with a4b1 volume (total water volume 750 m3.ha-1) with a decrease of 70.6% compared to the a2b3 volume (The total volume of water was 6000 m3.ha-1). Also, the results showed that the highest decrease in corm water use efficiency was obtained in the a1b4 treatment (total water volume 13750 m3.ha-1) decreased by 85.4 percent compared to a3b1 (total volume of water 1000 m3.ha-1) and the greatest decrease in fresh flower water use efficiency was shown in the a1b4 treatment (total volume of water 13750 m3.ha-1) decreased by 82.4% compared to a2b1 (total volume of water 1500 m3.ha-1). According to the results, the use of 250 to 500 m3.ha-1 irrigation in 30-day intervals (1500 to 3000 m3.ha-1) is recommended for maximum water use efficiency in saffron cultivation.
Agriculture
Mohammadreza Maleki; Mohammadjavad Seghatoleslami; Gholamreza Mousavi; Hassan Feizi
Abstract
This experiment was carried out as a split plot based on randomized complete block design with three replications in 2016-2017 and 2017-2018 and the data related to 2017-2018 were analysed. The irrigation time for the main plot (A) consisted of 4 levels including I1: control (traditional irrigation) ...
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This experiment was carried out as a split plot based on randomized complete block design with three replications in 2016-2017 and 2017-2018 and the data related to 2017-2018 were analysed. The irrigation time for the main plot (A) consisted of 4 levels including I1: control (traditional irrigation) on October 12, I2: irrigation at 10 days after I1, I3: I1+ one additional irrigation after the last irrigation (April 30), I4: the first irrigation time was done 10 days later than the traditional irrigation+ one additional irrigation 10 days after the last irrigation. Sub plot (B) was the foliar application of various stimulators including of G1: control, G2: acid salicylic, G3: Complete micro fertilizer, G4: nano Tio2, G5: nano Sio2. In this experiment different traits of corm and flower and stigma quality were determined. The results indicated that foliar application of complete micro fertilizer and Sio2 nanoparticles had the highest number of daughter corms. Moreover,both of them caused 25 percent increase overthe control group. Irrigation times at I4 and I1 yielded the maximum and minimum contents of dry weight of style and stigma. Foliar application of complete micro fertilizer was the best treatment in terms of dry weight of style and stigma. The most contents of crocin among various fertilizers belonged to complete micro and Tio2 nanoparticles. The highest amount of picrocrocin belonged to complete micro fertilizer and salicylic acid. The salicylic acid foliar application was the best treatment in terms of chlorophyll content of saffron leaves. In conclusion the first irrigation on October 12 and using complete micro fertilizer are reconded.