Biotechnology and genetics
faezeh gharari
Abstract
Saffron is grown for using its flower and red color stigma. This plant has many uses in the food industry, paints and medicine. Due to its high monetary worth, identification of genes involved in the flowering is very important. In order to better understand the molecular mechanisms involved in the onset ...
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Saffron is grown for using its flower and red color stigma. This plant has many uses in the food industry, paints and medicine. Due to its high monetary worth, identification of genes involved in the flowering is very important. In order to better understand the molecular mechanisms involved in the onset of flowering, Gene and gene expression of Pistillata-Like MADS box (PIC2) were studied. The Gene sequences have high homology with other family members of the MADS-box family (one of the transcription factors control the expression of proteins flowers). In this research, the gene was studied using bioinformatics resources. Primers were designed for amplification of the gene and then the total DNA and RNA were extracted from the leaves of saffron. cDNA synthesis was performed by the reverse transcriptase enzyme and it was used as a template for amplification of PIC2 gene by PCR reaction. The reaction was amplified to a fragment with length 900 bp from cDNA and another fragment with length 2100 bp from genomic DNA. This gene encodes a protein that includes 210 amino acids. By analyzing the molecular structure of protein transcripts based on modeling of the gene homology, the spatial figure showed that they have a regular structure.
Parviz Rezvani Moghaddam; Alireza Karbasi; Moein Tosan; Faezeh Gharari; Hassan Feizi; Toktam Mohtashami
Abstract
Khorasan province in Iran with a diverse climatic conditions has accommodated a wide range of plant communities particularly herbs, spices and medicinal plants. Among these Saffron (Crocus sativus L.), has been the most cultivated plant for thousands of years. These crop deliver unique interests ...
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Khorasan province in Iran with a diverse climatic conditions has accommodated a wide range of plant communities particularly herbs, spices and medicinal plants. Among these Saffron (Crocus sativus L.), has been the most cultivated plant for thousands of years. These crop deliver unique interests and applications. The novel use of Saffron in recent years in cancer cure have been promenaded and stimulated more investigation on this crop. Almost 94% of the total world’s Saffron production (298 t) and 95% of the total Iran’s Saffron production (280 t) originates from Khorasan province. Saffron is unique for the area where water scarcity is the most limiting factor in crop productions for the farmer. Saffron is almost cultivated and harvested and also to some extent processed by family workers and community cooperation bases. These crop is not only the most important source of income for farmers but also historically strong socio-cultural activities have been formed within the local community. Cultivation area and its surrounding environment conditions and production volume of Saffron, has made Khorasan province a unique location in the world. The sustainable management of these traditionally cultivated and used plants not only helps to conserve nationally and globally important biodiversity but also provides critical resources to sustain livelihoods.
