Biotechnology and genetics
Akbar Tavakkoli; Ali Sorooshzade; Majid Ghorbani Javid
Abstract
The objectives of this research were investigated the effects of removal of lateral and main buds in different corm size on vegetative traits and yield of saffron. The research was conducted as factorial based on a randomized complete block design with three replications at the Research field of Faculty ...
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The objectives of this research were investigated the effects of removal of lateral and main buds in different corm size on vegetative traits and yield of saffron. The research was conducted as factorial based on a randomized complete block design with three replications at the Research field of Faculty of Agriculture, Tarbiat Modares University in Tehran-Iran, during growing season of 2012-2013. The first factor was corm size with two levels of corm weight (2-4 and 6-8 g) and the second factor was buds removal with nine levels included without bud removal, remove all lateral buds, remove all buds except main buds and one lateral bud, remove all buds except main buds and two lateral buds, remove all buds except main buds and three lateral buds, remove all buds except one lateral bud, remove all buds except two lateral buds, remove all buds except three lateral buds, remove all buds except four lateral buds. In this research, vegetative traits in the first year and reproductive traits in the second year were investigated. The results showed that by removing main bud from corm leaf length and root length were decreased. Also, there was most root length by removing all lateral buds. Overall, the results showed that bud removal could be produce large corm but main bud should not eliminated. The most appropriate the number of buds that could be remaining is 4 large buds on saffron corm.
Biotechnology and genetics
Masoumeh Aliakbari; Rouhollah Shamloo-Dashtpagerdi; Esmaeil Ebrahimie
Abstract
Functional genomics methods such as Expressed Sequenced Tag (EST) analysis have provided possibilities for identification, expression analysis and study of transcripts involved in metabolic and regulatory networks. In order to identify of genome orientation and to determine gene networks involved in ...
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Functional genomics methods such as Expressed Sequenced Tag (EST) analysis have provided possibilities for identification, expression analysis and study of transcripts involved in metabolic and regulatory networks. In order to identify of genome orientation and to determine gene networks involved in the evolution of saffron stigma, 6202 EST sequences from mature saffron stigma were analyzed. After initial trimming, sequences clustering and assembling resulted in 910 unigenes (604 Contigs and 304 Singleton). BLAST X revealed that 570 unigene had significant hit among the Arabidopsis protein database, whereas the remaining unigenes displayed no significant match with the any hit. Classifying and gene enrichment analysis of unigenes, put them into 31 distinct functional groups, where 12 groups of them were statistically significant at α=0.01. Gene network of high represented Contigs (which had greater than 20 transcripts), showed that there is a complex gene interaction in mature saffron stigmas. Results revealed that jasmonic acid signalling pathway and its transcription factors such as MYB21 and Zinc fingers play a key role in regulating of stigma primary and secondary metabolism, especially in metabolism of carotenoids (as the most important saffron metabolites). The genes identified in this study could be good candidates for manipulating the evolution and metabolism of saffron stigma.
