Saffron Agronomy and Technology
In collaboration Iranian Medicinal Plants Society

Molecular detection of some plant and non-plant frauds in commercial saffron using ITS marker

Document Type : Research Paper

Authors

Nasrin Moshtaghi 1
Elham Hamidi 2
Abdolreza Bagheri 3
Ahmad Sharifi 4
Abbas Hemati Kakhki 5

1 Assistant professor, Faculty of Agriculture, Ferdowsi University of Mashhad

2 Professor, Biotechnology Research Center, Ferdowsi University, Mashhad

3 Msc Student of Agricultural Biotechnology, Ferdowsi University of Mashhad

4 Msc Student of Agricultural Biotechnology, Ferdowsi University, Mashhad

5 Iranian Academic Culture for Education, Culture and Research-Branch, Mashhad

https://doi.org/10.22048/jsat.2014.4816
Abstract
Saffron (Crocus sativus L.) is the most valuable food additive in the world which little production and high price of it caused some adulterations such as plant and chemical material similar to saffron. There are several methods for detecting these fraudulent based on morphological and chemical tests but they are not effective in some cases. In this research a novel molecular method based on ITS-2 marker is introduced. A common forward primer based on 5.8s rDNA for all plant frauds such as safflower, corn stigmas, pomegranate, turmeric and capsicum slices was designed then specific reverse primers based on ITS-2 for any frauds have been designed for polymerase chain reaction. Related ITS-2 bands were amplified in any adulterations in saffron. Specific primer for camel and cow meet fibers was designed based on cytochrome b gene and could amplified the related bands. Multiplex PCR with all of these primers could amplify all of the bands related to any adulterations. Furthermore, using 20% polyacrylamide gel lead to good segregation of bands. This method can be used successfully for detection of low percentage (1%) of fraudulent in saffron. So this marker can be used efficiently for detection of these frauds in commercial saffron.

Keywords

Fraud Saffron
ITS marker
Polymerase Chain Reaction

Subjects

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