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Identification of ISSR markers related to quantitative traits of Crocus Sativus L. ecotypes

Document Type : Research Paper

Authors

1 Assistant professor, Crop and Horticultural Science Research Department, Southern Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Jiroft, Iran

2 Professor of Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

3 Assosciate Professor, Department of Plant Breeding and Biotechnology, faculty of Agriculture, University of Tabriz, Iran.

4 Associate Professor of Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

Abstract

In order to investigate the relationship between quantitative traits and ISSR markers, an experiments were carried out under farm and laboratory conditions at Zanjan University. In this experiment, 20 primers of ISSR marker were used and the agronomic traits (including flower number, fresh weight of Stigma, fresh weight of flower, dry weight of Stigma, dry weight of flower, stigma length, saffron yield, corm number, feresh and dry weight of corm, number of leaf, leaf length, leaf width, leaf area, leaf dry weight, biomass, harvest index), physiological traits (transpiration rate, stomatal conductance, photosynthesis) and secondary metabolites (picocrocin, Safranal and Crocin) were measured during the growing season. Three primers of total did not amplified. 17 ISSR primers amplified 133 loci among 20 saffron ecotypes, with an average of 7.82 loci per primer. The highest number of alleles were for the I-8 primer (15 alleles).

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References
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Saffron Agronomy and Technology
Volume 8, Issue 4 - Serial Number 30
December 2020
Pages 598-608
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