Document Type : Research Paper

Authors

• Noraddin Hosseinpour azad ¹
• Ghorban Ali Nematzadeh ²
• Giovanni Giuliano ³
• Gholam Ali Ranjbar ⁴
• Ahad Yamchi ⁵

¹ Assist. Prof. of Planr breeding-Genetic Engineering, Meshkinshahr Faculty of Agriculture, University of Mohaghegh Ardabili, Meshkinshahr, Iran.

² Academic member at Department of Plant Breeding, Sari University of Agriculture, Sari, Iran

³ Prof., Plant Biology, Department of Biotechnology, Italian National Agency for New Technologies, Rome, Italy.

⁴ Academic member at Department of Plant Breeding, Sari University of Agriculture, Sari, Iran.

⁵ Assist. Prof. of Genetic Engineering and Molecular Genetics,Gorgan University of Agricultural Science and Natural Resources Golestan, Gorgan, Iran,

Abstract

The main metabolites in saffron are the Apo- carotenoids’ Crocin and Crocetin. Color intensity and quality of saffron mostly depend on the presence of Crocins that are glycosylated steric form of Crocetin by glycosyltransferase enzyme. The aim of this study is the characterization of these metabolites in methanolic and chloroformic extracts of saffron stigmas during anthesis stage by LC-APCI-MS. Identification of cis and trans isomers of Crocin and Crocetin was done by three parameters such as mass spectra registered in the negative ion mode, retention time and absorption ratio related to each metabolites. The variability of these parameters made it possible to detect the Crocins isomer with regard to the attached position and the number of UDP- glucose and Gentiobiosyl molecules to Crocetin structure. Crocins was the mainly detected components as there are polar components that are classified in the carotenoeids groups and the strified form of Crocetin Glucose (β-D-Glucopyranosyl) and Gentiobiose (β-D-Glucopyranosyl-D-Glucose). Also doubly charged ions were found for trans-isomers of Crocin-4, due to the high symmetry of their molecules. Based on the data gathered, the applied chromatograph Machin in this project is accurate and it is most sensitive tools to investigate about plants’ natural components like saffron, also the used APCI-MS in negative ions mode is the most efficient method to distinguish different steric forms of Crocin based on the ion’s fragments related to united reduction of glycosyl and gentiobiosyl as well as molecular fractions.

Keywords

• Saffron
• Metabolite
• Crocetin
• Crocin
• Mass spectrometry

20.1001.1.23831529.1395.4.4.4.2

Main Subjects

• Processing, food industry and biochemistry