Genetic Diversity of Alternaria alternata the Causal Agent of Saffron Rot in Southern and Razavi Khorasan Provinces Using Molecular Markers

Najari, Ghasem; Nourollahi, Khoshnood

doi:10.22048/jsat.2018.113230.1277

Document Type : Research Paper

Authors

¹ Master of plant protection department, Ilam University

² Associate professor of plant protection department, Ilam University

https://doi.org/10.22048/jsat.2018.113230.1277

Abstract

Alternaria leaf spot caused by Alternaria alternata is one of the most important plant diseases in world. In order to determine genetic diversity, 50 samples were collected from saffron farms of different regions in Razavi Khorasan province (Kashmar, Torbatjam, Torbathaidaryeh, Mahvellat, Bardeskan) and South Khorasan province (Qaen, Saraian, Brjand). A. alternata isolates were isolated with Potato Dextrose Agar media, Molecular test was carried out with a set of five pairs of SSR primers after purification and identification of isolates. A total of 22 alleles were produced by SSR primers with an average of 11.1 alleles in all populations. The highest amounts of alleles were related to locus Mavellat with 14 alleles and lowest of Saraian, Birjand and Qaen with 10 alleles. A Comparison of genetic diversity parameters in eight population showed that Torbatjam population has the highest genetic diversity but lower values were estimated for Qaenat. The highest and lowest genetic distance was detected between Qaen-Mahvellat (0.328) and Bardaskan-Mahvellat (0.054), respectively. Based on dendrogram of populations revealed two distinct groups, one group contained Qaen and the other Birjand, Mavellat, Saraian, Bardaskan, Kashmar, Torbatjam and Torbat hidaryeh. Analysis of molecular variance showed that 71 percent of the genetic diversity belongs to within populations and 29% is located among populations. There was the high genetic similarity between isolates from different regions. Results in this study will be useful in breeding for saffron resistant cultivars and developing necessary control measures.

Keywords

20.1001.1.23831529.1398.7.3.4.1

Main Subjects

Biotechnology and genetics

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Saffron Agronomy and Technology

Genetic Diversity of Alternaria alternata the Causal Agent of Saffron Rot in Southern and Razavi Khorasan Provinces Using Molecular Markers

References

References

Volume 7, Issue 3 - Serial Number 25
August 2019
Pages 331-346

Genetic Diversity of Alternaria alternata the Causal Agent of Saffron Rot in Southern and Razavi Khorasan Provinces Using Molecular Markers

References

References

Volume 7, Issue 3 - Serial Number 25August 2019Pages 331-346

Volume 7, Issue 3 - Serial Number 25
August 2019
Pages 331-346