Agriculture
Ali Hossainnia; Abbas Mohammadi
Abstract
Saffron is one of the most important herbs and spices in the East of Iran and its production is affected by various pathogens. This study is aimed at isolation, identification and pathogenicity detection of Alternaria alternata isolates as leave spot and corm rot of saffron agents. Soil samples and infected ...
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Saffron is one of the most important herbs and spices in the East of Iran and its production is affected by various pathogens. This study is aimed at isolation, identification and pathogenicity detection of Alternaria alternata isolates as leave spot and corm rot of saffron agents. Soil samples and infected plant tissues were collected from different areas of the Birjand plain (Birjand, Khusf and Sarbishe) during the years 2014-15. Alternaria species were isolated by potato dextrose agar medium and their morphological characteristics were studied on the potato carrot agar medium. For molecular studies, genomic DNA was extracted by the CTAB method and ribosomal subunits of fungal isolates were amplified using ITS1 and ITS4 primers. Based on morphological characteristics, 80 isolates of A. alternata were isolated. The ITS regions sequences of selected isolates had 100 similarities with A. alternata spices sequences in NCBI and MycoBank. These isolates caused chlorosis and necrosis on detached leaves, the inoculated corm was rotten and inoculated plants showed chlorosis and necrosis on leaves after 3-5 days. The results showed that A. alternata in the Birjand plain can cause corm rot and leaf chlorosis and necrosis is saffron. This study is the first report of the virulence of this fungus on saffron tissue in the East of Iran.
Agriculture
Zahra Heydari; Hossein Besharati; Saeideh Maleki Farahani
Abstract
In this research, effects of chemical and biological fertilizers on qualitative and quantitative traits of saffron, were evaluated in a field experiment as randomized complete block design with three replications at Saffron Research Farm of Shahed University during growing season of 2012-2013. Experimental ...
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In this research, effects of chemical and biological fertilizers on qualitative and quantitative traits of saffron, were evaluated in a field experiment as randomized complete block design with three replications at Saffron Research Farm of Shahed University during growing season of 2012-2013. Experimental factors were chemical nitrogen fertilizer (0, 25 and 50 kg.ha-1) and plant growth promoting rhizobacteria including Pseudomonas and Bacillus as biofertilizer (inoculation and uninoculation). The results indicated that complete application of chemical fertilizer with biofertilizer enhanced yield of saffron, up to 217%, compared to control. The highest yield obtained in application of biofertilizer and 50 kg.ha-1 chemical fertilizer. It is worth noting that control (no inoculation) resulted in highest percentage of ingredients of stigma. Application of 50 kg.ha-1 of fertilizer was caused severe loss of quality traits in stigma, as safranal and crocin contents decreased by 5.13% and 10.9%, respectively. Integrated application of 25 kg.ha-1 nitrogen fertilizer and bio-fertilizer increased the picrocrocin up to 11.9% compared to control. Application of 50 kg.ha-1 nitrogen fertilizer along with biofertilizer was the efficient treatment in increment of stigma yield and qualitative characteristics of saffron leaves. The concentration of phosphorus, zinc and copper increased up to 83.05, 69.36 and 86.6%, respectively compared to control. Totally, Pseudomonas and Bacillus inoculation increased most of the qualitative and quantitative traits of saffron through nutrients uptake.
