Saffron Agronomy and Technology

Saffron Agronomy and Technology

Evaluation the inhibitory effects of chemical compounds for controlling on Burkholderia gladioli pv. gladioli, the causal agent of saffron corm rot

Document Type : Research Paper

Authors
Maryam Khezri 1
MAHMOUDREZA KARIMISHAHRI 2
Abolghasem Ghasemi 3
1 Assistant Professor, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
2 RAZAVI-KHORASSAN AGRRICULTURAL AND NATURAL RESOURCES RESEARCH CENTER PLANT PROTECTION DEPTT.
3 Assistant professor, Agricultural Education and Extension Research Organization, Iranian Plant Protection Research Institute, Tehran, Iran
10.22048/jsat.2024.454510.1529
Abstract
Saffron corm rot caused by the bacterium Burkholderia gladioli pv. gladioli (Bgg), is an important disease affecting saffron cultivation. This study aimed to evaluate the effect of different doses of sodium bicarbonate and three copper-based compounds (copper oxychloride, Bordeaux and Nordox) on the population of Bgg isolates, in vitro. Additionally, the sensitivity of the Bgg isolates to 21 antibiotics was investigated. Two weeks after inoculation, the symptoms of the disease appeared as dry rot and brown lesions around the inoculated area. Varying degrees of virulence were observed among the Bgg isolates, with the Bg16 inducing the most severe symptoms. The Minimum inhibitory concentrations (MICs) were determined as copper oxychloride 3.5/1000, Bordeaux and sodium bicarbonate 12%, and Nordox 1.5/1000. The Bgg isolates were sensitive to nine antibiotics. The largest inhibitory zone diameters observed around trimethoprim sulfamethoxazole, nalidixic acid, gentamicin, and amikacin discs. One week after cultivation, the copper compounds decreased the bacterial population compared to the control, but no significant difference was observed between them in the second week. The most effective treatments in reducing the Bgg population were copper oxychloride at 2/1000 and 3/1000, Bordeaux at 8% and 10%, and Nordox at 1/1000 and 1.5/1000. The efficacy of the identified effective treatments will be further evaluated under field conditions.
Keywords
Saffron
Disinfestation
Copper compounds
Antibiotic
Disease management

Subjects

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