Saffron Agronomy and Technology

Saffron Agronomy and Technology

Evaluation of Fulzyme Plus-SP Biological Fertilizer and Aminofol Biostimulant on Some Vegetative and Reproductive Traits of Saffron (Crocus sativus L.)

Document Type : Research Paper

Authors
Mina Gholampour 1
Hossein Sadeghi 2
Vahid Akbarpour 3
1 Former M.SC. Student of Medicinal Plants, Department of Horticultural Sciences and Engineering, Sari Agricultural Sciences and Natural Resources University, Sari
2 Associated Professor of Horticultural Sciences and Engineering Department; respectively, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
3 Assistant Professor of Horticultural Sciences and Engineering Department; respectively, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
10.22048/jsat.2024.465316.1534
Abstract
Saffron Yield and flowering depend on several factors, including the size of the corms. The production of daughter corms and the yield of flowers depend on the size of the mother corms. Large corms produce more corms and flowers per unit surface. In this investigation, the effect of Aminoful and Fulzyme Plus-Sp fertilizers simultaneously on the size of the corms and the yield of stigmas were studied. This experiment was conducted in 2018- 2019 on a saffron farm in Farim city 65 kilometers away from Sari city, with an altitude of 818 meters above sea level in a factorial form based on a randomized complete block design with nine treatments and three replications. In this research, saffron corms were prepared from Torbat- Jam landrace. Study treatments included Aminoful biostimulant (0, 125 and 250 mL.L-1) and Fulzyme Plus-SP biological fertilizer (0, 2.5 and 5 g.L-1).  The results showed that the interaction effect of aminoful foliar and application of Fulzyme plus-SP biofertilizer on the fresh weight of the leaf was significant at p<0.01, although the effect of these treatments on the number of leaves, dry weight of leaf, fresh weight of the daughter corms, the diameter of the daughter corms, fresh weight of flower and petals were significant at p< 0.05. The maximum number of leaves, fresh weight of leavesand dry weight of leaveswere observed using Fulzyme Plus-SP biological fertilizer and Aminofol biostimulant (A2F2). Also, the highest fresh weight of daughter corms and diameter of daughter corms after usage of Fulzyme Plus-SP biofertilizer and biostimulant Aminoful (A2F1) 57.71% and 22.56% respectively increased compared to the control one. The maximum fresh weight of flowers and petals was discovered with the application of Fulzyme Plus-SP and biostimulant Aminofol (A2F1 biological fertilizers. Moreover, these biological fertilizers had a positive impact on the morphological characteristics of saffron leaves and corms, causing an increasing the photosynthetic organs and growth of daughter corms. Therefore, the using of these two compounds led to an increase in corms weighing more than 20 grams. Also, these fertilizers increased the flowers and stigmas of saffron by more than 30% compared to not using them in the next year by increasing the number and size of saffron corms.
Keywords
Amino acid
Foliar nutrition
Saffron
Stigma Yield
Biostimulant

Subjects

Agricultural statistics (Horticultural products), (2019). Ministry of Agricultural Jihad.
Alizadeh-Salteh, S. (2015). Evaluation of the effect of maternal corm and planting methods on flower and replacement corms yield of two ecotypes of saffron (Crocus sativus L.) in Tabriz. Saffron Agronomy & Technology, 3 (4), 251-259. (In Persian with English Abstract) https://doi.org/10.22048/jsat.2016.11762
Amini, S., Maleki Farahani, S., Sharghi, Y., & Zahedi, H. (2014). Influence of vermicompost and bacterium of Bacillus and Pseudomonas on growth, yield and morphological traits of saffron. Applied Science & Agriculture, 9 (3), 933-941. https://doi.org/10.13140/RG.2.1.2571.1849
Asadi, G. A., Khorramdel, S., Ghorbani, R., & Bicharanlou, B. (2019). Evaluation of the effect of soil fertilizers and summer irrigation on corm and flower yield of saffron. Saffron Agronomy & Technology, 6 (4), 393-414. (In Persian with English Abstract) https://doi.org/10.22048/jsat.2018.70724.1205
Behdani, M A., Koocheki, A., Nassiri, M., & Rezvani, P. (2005). Evaluation of quantitative relationships between saffron yield and nutrition (on farm trial). Iranian Journal of Field Crops Researches, 3 (1), 1-14. (In Persian with English Abstract) https://doi.org/10.22067/GSC.V3I1.1287
Behnia, M.R., & Mokhtari, M. (2009). Effect of planting methods and corm density in saffron (Crocus sativus L.) yield. Acta Horticulturae, 850, 131-136. https://doi.org/10.17660/ActaHortic.2010.850.20
Chaji, N., Khorassani, R., Astaraei, A.R., & Lakzian, A., (2013). Effect of phosphorous and nitrogen on vegetative growth and production of daughter corms of saffron. Journal of Saffron Research, 1 (1), 1-12. (In Persian with English Abstract) https://doi.org/10.22077/jsr.2013.352
Chen, J. (2006). The combined use of chemical and organic fertilizers and or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient Crop Production and Fertilizer Use. October, 16-20. Thailand. 11p.
Dole, J. M., & Wilkins, H. F. (1999). Floriculture principles and species. Prentice Hall, 537-545.
Elser, J. J. (2012). Phosphorus: a limiting nutrient for humanity. Current Opinion in Biotechnology, 23, 833-838. https://doi.org/10.1016/j.copbio.2012.03.001
Emami, M., Armin, M., & Jamimoeini, M. (2018). The effect of foliar application time of organic and chemical fertilizers on yield and yield components of saffron. Saffron Agronomy & Technology, 6 (2), 167-179. (In Persian with English Abstract). https://doi.org/10.22048/jsat.2017.72599.1211
Fageria, N. K. (2007). Yield physiology of rice. Journal of Plant Nutrition, 30, 843-879. https://doi.org/10.1080/15226510701374831
Fallahi, J., Koocheki, A.R., & Rezvani Moghaddam, P. (2009). Effects of biofertilizers on quantitative and qualitative yield of chamomile (Matricaria recutita) as a medicinal plant. Iranian Journal of Field Crops Research, 7 (1), 127-135. (In Persian with English Abstract).  https://doi.org/20.1001.1.20081472.1388.7.1.13.1
Faten, S. A., Shaheen, A. M., Ahmad, A. A., & Mahmoud, A. R. (2010). Effect of foliar application of amino acids as antioxidants on growth, yield and characteristics of squash. Research Journal of Agriculture & Biological Science, 6 (5), 583-588.
Fiori, M., Falchi, G., Quaglia, M., & Cappelli, C. (2007). Saffron (Crocus sativus L.) diseases in Italy. Plant Pathology, 89, 27-68. https://doi.org/10.22059/IJHS.2021.319185.1898
Ghasemi-Rooshnavand, R., Hashemiyeh, M., & Afzalian, M. (2009). Planting, conservation and harvesting stages of saffron. Yazd Agriculture Organization, P33. (In Persian with English Abstract)
Ghavi, M., & Sadrabadi Haghighi, R. (2015). Effect of the foliar application of biological, biostimulation, and chemical fertilizers on growth characteristics and yield of saffron (Crocus sativus L.). Journal of Crop Production, 8 (3), 139-158. (In Persian with English Abstract) https://doi.org/20.1001.1.2008739.1394.8.3.8.2
Gholami, A., Shahsavani, S., & Nezarat, S. (2009). The effect of Plant Growth Promoting Rhizobacteria (PGPR) on germination, seedling growth and yield of maize. International Journal of Biological & Life Science, 1 (1), 35-40.  https://doi.org/10.5281/zenodo.1083384
Gholami, M., Kafi, M., & Khazaei, H.R. (2017). Study of the relations of sink and source in saffron by means of correlation coefficients under different irrigation and fertilization levels. Saffron Agronomy & Technology, 5 (3),195- 210. (In Persian with English Abstract) https://doi.org/10.22048/jsat.2017.51480.1156
Gull, M., Hafee, F.Y., Saleem, M., & Malik, K. (2004). Phosphorus uptake and growth promotion of chickpea by co- inoculation of mineral phosphate solubilising bacteria and mixed rhizobial culture. Australian Journal of Experimental Agriculture, 44, 623-628. https://doi.org/10.1071/EA02218
Gutierrez-Manero F.J., Ramos-Solano, B., Probanza, A., Mehouachi, J., Tadeo, F.R., & Talon, M. (2001). The plant-growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiologia Plantarum, 111, 206–211. https://doi.org/10.1034/j.1399-3054.2001.1110211.x
Haj Seyed Hadi, M. R., & Rezaee Ghale, H. (2016). Effects of vermicompost and foliar application of amino acids and urea on quantitative and qualitative yield of chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal & Aromatic Plants, 31 (6), 1058-1070. (In Persian with English Abstract) https://doi.org/10.22092/ijmapr.2016.105894
Hassanzadeh Aval, F., Rezvani Moghaddam, P., Bannayan aval, M., & Khorasani, R. (2013). Effects of maternal corm weight and different levels of cow manure on corm and flower yield of saffron (Crocus sativus L.). Saffron Agronomy & Technology, 1 (1), 22-39. (In Persian with English Abstract) https://doi.org/10.22048/jsat.2013.4809
Khatami, F., EsmaiElpour, B., Hadian, J., Khavazi, K., Soltani, A., & Abbaszadeh-dahaji, P. (2014). Effects of Biofertilizers on Morphological Traits of Tarragon‏. Journal of Soil Biology, 1 (2), 56-63. (In Persian with English Abstract). https://doi.org/10.22092/SBJ.2014.100091
Heydari, Z., Besharati, H., & Maleki Farahani, S. (2014). Effect of some chemical fertilizer and biofertilizer on quantitative and qualitative characteristics of Saffron. Journal of Saffron Agronomy & Technology, 2 (3), 177-189. (In Persian with English Abstract).
Kafi, M., Rashed Mohassel, M. H., Koocheki, A., & Mollafilabi, A. (2002). Saffron Production and Processing. Zaban va Adab Press, Iran.
Karima, M., Gamal EL-Din, M., & Abdel-Wahed, M. S. A. (2005). Effect of some amino acids on growth and essential oil content of chamomile plant. International Journal of Agriculture & Biology, 3, 376–380.
Kaushal, S.K., & Upadhyay, R.G. (2002). Studies on variation in corm size and its effect on corm production and flowering in Crocus sativus L. under mid hill conditions of HP. Research on Crops, 3 (1), 126-128.
Khandan Deh Arbab, S., Aminifard, M. H., Fallahi, H. R., & Kaveh, H. (2020). Effect of different levels of novafol bio-fertilizer and mother corm weight on vegetative growth, flowering and chlorophyll content of saffron. Saffron Agronomy & Technology, 7 (4), 441-455. (In Persian with English Abstract). https://doi.org/10.22048/jsat.2019.133954.1303
Kheiry, A., Parsa, H., Sani Khani, M., & Razavi, F. (2018). Effect of bio-fertilizers and nitrogen on quantitative and qualitative characteristics of petal in saffron (Crocus sativus L.). Saffron Agronomy & Technology, 6 (3), 309-322. (In Persian with English Abstract). https://doi.org/10.22048/jsat.2017.92549.1246
Koocheki, A., Ganjeali, A., & Abbassi, F. (2006). The effect of duration and condition of incubation, weight of mother corms and photoperiod on corm and shoot characteristics of saffron plant (Crocus sativus L.). Iranian Journal of Field Crops Researches, 4 (2), 331-315 (In Persian with English Abstract). https://doi.org/10.22067/GSC.V4I2.1271
Koocheki, A., Nassiri, M., Alizadeh, A.,  & Ganjeali, A. (2010). Modelling the impact of climate change on flowering behaviour of Saffron (Crocus sativus L.). Iranian Journal of Field Crops Researches, 7 (2), 583-594. (In Persian with English Abstract). https://doi.org/20.1001.1.20081472.1388.7.2.25.5
Koocheki, A., & Seyyedi, S. M. (2015). Relationship between nitrogen and phosphorus use efficiency in saffron (Crocus sativus L.) as affected by mother corm size and fertilization.  Industrial Crops & Products, 71, 128–137. https://doi.org/10.1016/j.indcrop.2015.03.085
Kudoyarova, G. R., Vysotskaya, L. B., Arkhipova, T. N., Kuzmina, L. Y., Galimsyanova, N. F., Sidorova, L. V., Gabbasova, I. M., Melentiev, A. I., & Veselov, S. Y. (2017). Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiologiae Plantarum, 39, 253. https://doi.org/10.1007/s11738-017-2556-9
Loper, J.E., & Schroth, M. N. (1986). Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. Physiology & Biochemistry, 76, 386-389. https://doi.org/10.1094/Phyto-76-386
Mahmoudi, H., & Alizadeh, Kh. (2012). Investigation of the effects of foliar application of free amino acids on quantitative and qualitative yield of chickpea (Jam cultivar) in dryland conditions. National Rainfed Agricultural Research Institute, 41 p. (In Persian)
Marulanda, A., Barea, J. M., & Azcon, R. (2009). Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. Plant Growth Regulation, 28, 115-124. https://doi.org/10.1007/s00344-009-9079-6
Mashayekhi, K., Soltani, A., & Kamkar, B. (2006). The relationship between corm weight and total flower and leaf numbers in saffron. Proceedings of the 2nd International Symposium on Saffron Biology and Technology. Mashhad, Iran 28-30 October, 93-96. (In Persian with English Abstract) https://doi.org/10.17660/ActaHortic.2007.739.11
Molina, R. V., Valerro, M., Navarro, Y., Guardiola, J. L. and Garcia-Luis, A. (2005). Temperature effects on flower formation in saffron (Crocus sativus L.). Journal of Scince Horticulture, 103 (3), 361-379. https://doi.org/10.1016/j.scienta.2004.06.005
Mollafilabi, A. (2004(. Experimental finding of production and ecophysiological aspects of saffron (Crocus sativus L.). Acta Horticulture, 650, 195–200. https://doi.org/10.17660/ActaHortic.2004.650.20
Muscolo, A., Bovalo, F., Gionfriddo, F., & Nardi, F. (1999). Earthworm humic matter produces auxin-like effects on Daucus carota cell growth and nitrate metabolism. Soil Biology & Biochemistry, 31, 1303-1311. https://doi.org/10.1016/S0038-0717(99)00049-8
Naghdi Badi, H., Omidi, H., Golzad, A., Torabi, H., & Fotookian, M. H. (2011). Change in crocin, safranal and picrocrocin content and agronomical characters of saffron (Crocus sativus L.) under biological and chemical of phosphorous fertilizers. Journal of Medicinal Plants, 10 (40), 58-68. (In Persian with English Abstract) https://doi.org/20.1001.1.2717204.2011.10.40.7.4
Nassiri Mahallati, M., Koocheki, A., Boroumand Rezazadeh, Z., & Tabrizi, L. (2008). Effects of corm size and storage period on allocation of assimilates in different parts of saffron plant (Crocus sativus L.). Agricultural Research of Iran, 5 (1), 155-166. (In Persian with English Abstract)
Nieto, K. F., & Frankenberger, W. T. (1989). Biosynthesis of cytokinins in soil. Soil Science Society of America Journal, 53, 735-740. https://doi.org/10.2136/sssaj1989.03615995005300030016x
Omidi, H., Naghdi Badi, H., Golzad, A., Torabi, H., & Footoukian, M. (2009). The effect of chemical and bio-fertilizer sources of nitrogen on qualitative and quantitative yield of saffron (Crocus sativus L.). Journal of Medicinal Plants, 8 (30), 98-109. (In Persian with English Abstract) https://doi.org/20.1001.1.2717204.2009.8.30.11.4
Owen, A. G., & D. L. Jones. (2001). Competition for amino acids between wheat roots and rhizosphere microorganisms and the role of amino acids in plant N acquisition. Soil Biology & Biochemistry, 33, 651-657. https://doi.org/10.1016/S0038-0717(00)00209-1
Pandey, D., Pandey, V. S., & Srivastava, R. P. (1979). A note on the effect of the size of corms on the sprouting and flowering of saffron. Progressive Horticulture, 6, 89–92.
Parray, J. A., Kamili1, A. N., Reshi, Z. A., Hamid, R., & Qadri, R. A. (2013). Screening of beneficial properties of rhizobacteria isolated from Saffron (Crocus sativus L.) rhizosphere. African Journal of Microbiology Research, 7 (23), 2905-2910. https://doi.org/10.5897/AJMR12.2194
Pouryousef Miandoab, M. and Shahravan, N. (2014). Effect of foliar application of Amino Acids at different times on yield and yield components of maize. Crop Physiology Journal, 6, 21-32. (In Persian with English Abstract) https://doi.org/20.1001.1.2008403.1393.6.23.2.6
Probanza, A., Lucas-Garcia, J. A., Ruiz Palomino, M., Ramos, B., & Gutierrez-Manero, F. J. (2002). Pinuspinea L. seedlings growth and bacterial rhizosphere structure after inoculation with PGPR Bacillus (B. licheniformis CECT 5106 and B. pumilus CECT 5105). Applied Soil Ecology, 20, 75–84. https://doi.org/10.1016/S0929-1393(02)00007-0
Rees, A. R. )1988(. Saffron- an expensive plant product. The Plantsman, 9 (4), 210-217.
Renau-Morata, B., Nebauer, S. G., Sanchez, M., & Molina, R. V. (2012). Effect of corm size, water stress and cultivation conditions on photosynthesis and biomas spartitioning during the vegetative growth of saffron (Crocus sativus L.). Industrial Crops and Product, 39, 40-46. https://doi.org/10.1016/j.indcrop.2012.02.009
Rezakhani, A., &  Haj Seyed Hadi, M. (2017). Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum L.). Iranian Journal of Field Crop Science, 48 (3), 777-786. (In Persian with English Abstract) https://doi.org/10.22059/IJFCS.2017.210745.654144
Salehi, M., Abutalebi, A.H., & Mohammadi, A.H. (2013). Effect of foliar application of amino calcium on fruit firmness and storage life of Golden Delicious apples. Life Sciences Journal, 10, 140-142. https://doi.org/10.15547/tjs.2017.01.003
Seyyedi, M.,  Khajeh-Hosseini, M., Rezvani Moghaddam, P., & Shahandeh, H. (2015). Relation between the increasing soluble phosphorus and nitrogen uptake and its effects on phosphorus harvest index of black seed. Iranian Journal of Field Crops Sciences, 46 (1), 25-36. (In Persian with English Abstract) https://doi.org/10.22059/IJFCS.2015.54043
Sharaf-Eldin, M. A., Elkholy, S., Fernandez, J. A., Junge, H., Cheetham, R. D., Guardiola, J. L., & Weathers, P. J. (2008). The effect of Bacillus subtilis FZB24 on flowers quantity and quality of saffron (Crocus sativus L.). Planta Medica, 74, 1316-1320. https://doi.org/10.1055/s-2007-987387
Wani, S. A., Chand, S., Wani, M. A., Ramzan, M., & Hakeem, K. R. (2016). Azotobacter chroococcum–A Potential Biofertilizer in Agriculture: An Overview. In Soil Science: Agricultural & Environmental Prospectives, 333-348. https://doi.org/10.1007/978-3-319-34451-5