In collaboration Iranian Medicinal Plants Society

The effect corm priming time with different levels of gibberellic acid on quantitative and qualitative traits of saffron (Crocus sativus L.) in the first year

Document Type : Research Paper

Authors

1 PhD student in Agrotechnology-Physiology of Crop Plants, Faculty of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.

2 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.

3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.

4 PhD in Agronomy-Crop Ecology, Faculty of Agriculture and Natural Resources, University of Tehran, University of Tehran, Tehran, Iran

Abstract
One of the methods to improve production and control flowering in plants is the use of growth regulators such as gibberellic acid. In order to investigate the effect corm priming time of saffron with gibberellic acid (GA3), a factorial experiment was conducted in a randomized complete block design with three replications at the Agricultural Research Station,University of Tehran in 2023. Two planting times (late June, late September) and four levels of gibberellic acid (0, 100, 200, and 400 ppm) were considered as treatments in the form of corm priming. The evaluated traits were the number of buds, time to germination, number of flowers, time to flowering, bud-to-flower conversion ratio, dry weight of stigma, and quality traits of saffron stigma. The results showed that priming saffron corms with 100 ppm gibberellic acid in late June significantly (α=1%) increased the number of flowers, bud-to-flower conversion ratio, fresh and dry weight of stigma, crocin and safranal. However, corm priming in late September with 200 ppm gibberellic acid resulted in a significant increase (α=1%) in the number of buds, number of flowers, stigma length, fresh and dry weight of stigma, and a significant decrease (α=1%) in the time to flowering onset and germination, although in some traits they were in the same statistical group as the 400 ppm level. Overall, the results of this experiment showed that first, corm priming in late June with low levels of gibberellic acid is better, but, corm priming in late September with higher levels is beneficial. Secondly, corms priming in late June and at a level of 100 ppm of the hormone can increase the amount of crocin and safranal and yield traits, while priming corms in late September can reduce the time to flowering and time to germination And will improve flower and corm yield in the first year.

Keywords

Subjects

Abrishami, M. (2004). Knowing Iran’s Saffron. Tosan Press, Tehran, Iran 320 pp. (In Persian).
Ameri, R.,  Azizi, M., & Mollafilabi, A. (2019). Effects of gibberellic acid and naphthalene acetic acid on saffron plant (Crocus sativus L.) under field conditions. Journal of Applied Horticulture, 21 (2), 123–130. https:// doi.org/340601811/Applied Horticul.2019
Aminifard, M. H., Shakeri, M.,  Behdani, M. A., & Tabatabaei.,  S. J. (2024). Effects of gibberellic acid and plant density on antioxidant activity and secondary metabolites of saffron (Crocus sativus L.). Journal of Saffron Research (semi-annual), 12 (1), 51- 64. (In Persian with English abstract). http://doi.org/10.22077/jsr.2021.4318. .1159.
Amirian, M., & Kargar, S. M. A. (2016). Evaluation of gibberellic acid, corm size and phosphoric fertilizer on yield and other traits of saffron (Crocus sativus L.) (Ghaen Ecotype). Journal of Saffron Research, 4 (1), 134-148. (In Persian with English abstract).https://doi.org/10.22077/jsr.2016.398.
Azizbekova, N. S., Miliyaeva, E. L., Lobovaand, N. V., & Chailakhyan, M. K. (1978). Effects of gibberllin and kinetin on formation of flower organs in saffron. Journal of Plant Physiology, 25 (3), 471-476.
Alvarezorti, M., Gomez Gomez, L., Rubio, J., Escriban pardo, J., Jimenez, F., & Fernandez, J. A. (2004). Development and gene expression in saffron corms. Journal of Acta Horticulture, 650, 141-148. https://doi.org/10.17660/ActaHortic.2004.650.14.
Behdani, M. A., & Fallahi, H. R. (2015). Technical Knowledge Based on Research Approaches. University of Birjand Press, Birjand, Iran, 411 p. (In Persian). https://doi.org/ 10.1515/plass.2015.0025.
Birader, G., & Navalagatti, C. M. (2008). Effect of plant growth regulators on physiology and quality in bitter gourd (Momordica charantia). University of Agricultural Sciences. MSc. Thesis. Dharwad.
Chao, W.S., Foley, M.E., Horvath, D.P, and Anderson, J.V. (2007). Signals regulating dormancy in vegetative buds. Journal of Plant Biology, 1, 49–56. https://doi.org/228341880/Plant biol.2007.
El-Naggar, A. H., El-Naggar, A. A. M., Ismaiel, N. M. (2009). Effect of phosphorus application and gibberellic acid on the growth and flower quality of (Dianthus caryophyllus L.). American Eurasian Journal of Agriculture and Environmental Science, 6 (4), 400-410.
Fallahi, H. R., Paravar, A., Behdani, M. A., Aghhavani-Shajari, M., & Fallahi, M. J. (2014). Effects of saffroncorm and leaf extracts on early growth of some plants to investigate the possibility of using them as associated crop. Notulae Scientia Biologica, 6 (3), 282-287. https://doi.org/9259/ Notulaebiologicae.2014.
Feizi, H., Moradi, R. (2019). Assessing involved Managing factors in gap yield between traditional and ideal saffron cultivating systems in Razavi and South Khorasan provinces. Journal of Saffron Research (semi-annual), 7 (2), 283-298. (In Persian with English abstract). http://dx.doi.org/10.22077/jsr.2019.2242.1089.
Ghobadi, F., Ghorbani Javid, M., & Sorooshzadeh, A. (2015). Effects of planting date and corm size on flower yield and physiological traits of saffron (Crocus sativus L.) under Varamin plain climatic conditions. Journal of Saffron Agronomy & Technology, 2, 265-276. (In Persian with English abstract). https://doi.org/10.22048/jast.2015.8620.
Ghorbani Javid, M., Sadat Hoseinifard, M., Allahdadi, I., & Soltani, E. (2022). Hormonal priming with BAP and GA3 induces improving yield and quality of saffron flower through promotion of carbohydrate accumulation in corm. Journal of Plant Growth Regulation, 41, 205–215. https://doi.org//348588631/Plant groth reguha.2022.
Hajisamadi asl, B., Hassanpouraghdam, M. B., & Khalighi, A. (2011). Effects of gibberellic acid (GA3) foliar application on growth characteristics and essential oil of lavender (Lavandula officinalis Chaix.). Journal of Agricultural Science & Sustainable Production, 2 (21), 24-32. (In Persian with English abstract).https://doi.org/JR_SUST-21-2_003
Hassanpour Asil, M., Mortazavi, H., Hatamzadeh, A., & Qasemnejad, M. (2012). The effect of gibberellic acid and calcium in reducing the growth period of iris (Iris hollandica var. Blue Magic) in the greenhouse and increasing the shelf life of cut flowers. Science & Technology of Greenhouse Crops, 3 (1), 63-70. (In Persian with English abstract).https://doi.org/20.1001.1.20089082.1391.3.1.5.3.
Hoseinifard,  M., Ghorbani Javid, M.,  Allahdadi, I., & Soltani, E. (2018). The Effect of hormone priming and corm weight on the yield of flowers and characteristics of daughter corms of saffron in the first year. Saffron Agronomy & Technology, 6 (1), 3-15.  (In Persian with English abstract). https://doi.org/  10.22048/jsat.2017.62317.1196.
Jing, H., Yuping, L.,Xiaohui, T., Huajing., R.,Chaoxiang, R., Jiang, C., Qinghua, W., Yi, J., Fuchang, G., & Jin, P. (2020). Transcriptome profiling of the flowering transition in saffron (Crocus sativus L.). Nature Research/Scientific Reports, 1-15. https://doi.org/10.1038/s41598-020-66675-6.
INS (Iran National Standard). (2006). Research Institute of Standard and Iran. Saffron Bulletin, No. 259. (in Persian).
Kabir, K. (2011). Effect of GA3, CCC and MH on vegetative growth, flower yield and quality of Chrysanthemum, Journal of Experimental Agriculture International, 2 (1), 17– 20.
Kafi, M., Zand, E., Kamkar, B., Shareefee, H. R., & Goldanee, M. (2003). Plant Physiology. Mashhad Jihad. Daneshgahi Press. Mashhad, Iran. 379p. (In Persian)
Kumar, V., Kumar, V., Umrao, V., & Singh, M. (2012). “Effect of GA3 and iaa on growth and flowering of carnation, HortFlora Research Spectrum, 1 (1), 69–72. https://doi.org/10. 5555/20183312072.
Koul, K. K., & Farooq, S. (1984). Growth and differentiation in the shoot apical meristem of saffron plant. Journal of Indian Botanical Society, (63), 153-169.
Koocheki, A., Nassiri, M., Alizadeh, A., & Ganjeali, A. (2009). Modelling the impact of climate change on flowering behavior of Saffron (Crocus sativus L.). Iran. Journal of Field Crop Research, 7 (2), 583-594. (In Persian with English abstract). https://doi.org/119160/75313880224.
Koocheki, A., Rezvani Moghaddam, P., & Fallahi, H. R. (2016). Effects of planting dates, irrigation management and cover crops on growth and yield of saffron. Journal of Agroecology, 3, 435-451. (In Persian with English abstract). https://doi.org/ 10.5555/20173313621.
Leite, V. M., Rosolem, C. A., & Rodrigues, J. D. (2003). Gibberlin and Cytokinin effects on soybean growth. Scientia Agricola, 60, 537-541.
Long, R. L., Williams, K., Griffiths, E. M., Flematti, G. R., Merritt, D. J., Stevens, J. C., Turner, S. R., Powles, S. B., & Dixon, K. W. (2010). Prior hydration of Brassica tournefortii seeds reduces the stimulatory effect of karrikinolide on germination and increases seed sensitivity to abscisic acid. Annals of Botany, 105, 1063- 1070. https://doi.org//20348089.2010.
Mansuri Isfahani, S. M., Mortezanejad, M., & Naderi Darbaghshahi, M. R. (2013). Impact of Giberlic acidon concentration of crocin, picrocrocin and safranal in Crocus sativus L. International Journal of Agriculture & Crop Sciences, 6 (1), 1000-1003. https://doi.org/ 10.22077/JSR.2021.4318.1159.
Ministry of Agriculture. (2019). Details of the implementation of the saffron support purchase plan in 1998. Deputy of Agricultural Affairs, Saffron Performance Report in Iran, 3 p. (In Persian).
Mzabri, I.,  Rimani, M., Kouddane, N.,  & Berrichi,  A. (2021). Study of the effect of pretreatment of corms by different concentrations of Gibberellic Acid and at different periods on the growth, flowering, and quality of saffron in Eastern Morocco. Hindawi Advances in Agriculture Volume 2021, Article ID 9979827, 12 p. https://doi.org/10.1155/2021/9979827.
Nassiri Mahallati, M., Koocheki, A., Boroumand Rezazadeh, Z., & Tabrizi, L. (2007). Effects of corm size and storage period on allocation of assimilates in different parts of saffron plants (Crocus sativus L.). Iranian Journal of Field Crops Research, 5 (1), 155-166 (In Persian with English abstract).https://doi.org/10.22048 /jsat.2015.10386.
Nekahi, M. Z., Soltani, A., Siahmarguee, A., & Bagherani, N. (2014). Yield gap associated with crop management in wheat (Case study: Golestan province-Bandar-gaz). EJCP, 7 (2), 135-156. (in Persian with English Abstract). https://doi.org/p1305980. 
Nazarbeigi, E., Fallahi, H. A., Naseri, R., & Mirzaei, A. (2014). Investigation of the effect of different concentrations of sodium chloride, salicylic acid and gibberellic acid on the content proline and chlorophylls a and b in two canola cultivars (Haval 104 and RGS). Applied Research in Plant Ecophysiology, 1 (2), 25-37. (In Persian with English abstract).http://doi.org/603296/jr-arpe-1-2-002.
Pazoki, A., Karimi, M., & Fooladi, A. (2010). Effect of planting dates on the yield of saffron (Crocus sativus L.) ecotypes in Natanz. Journal of Journal o f Crop Physiology, 2, 3 -12. (In Persian with English abstract). https://doi.org./10.13870/journal.1389.
Pazoki, A., Karaminejad, M., & Foladi Targhi, A. (2011). Effects of planting dates and genotypes on yield of Saffron. Crop Physiology Journal, 2 (8), 3-28. (In Persian with English abstract). https://doi.org/174495/26213890801.
Phengphachanh, B., Naphrom, D.,  Bundithya, W. & Potapohn, N. (2012). Effects of day-length and gibberellic acid (GA3) on flowering and endogenous hormone levels in Rhynchostylis gigantea (lindl.) ridl, Journal of Agricultural Science, 4 (4), 217–222.
Renau-Morata, B., Nebauer, S. G., Sánchez, M., & Molina, R. V. (2012). Effect of corm size, water stress and cultivation conditions on photosynthesis and biomass partitioning during the vegetative growth of saffron (Crocus sativus L.). Indian Crop Product, 39, 40-46. https://doi.org//S092666901200088x.
Sadeghi, B. (1996). effect of warehousing and planting dates on flowering corms of saffron. Iranian Research Organization for Science and Technology, Khorasan Institute.
Sajjad, Y., Jaskani, M., & Qasim M. (2015). Pre-plant soaking of corms in growth regulators influences the multiple sprouting, floral and corm associated traits in Gladiolus grandiflorus L. Journal of Agricultural Science, 7 (9), 173–181.
Singh, D., Sharma, S., Jose-Santhi, J., Kalia, D., & Singh, R. K. (2023). Hormones regulate the flowering process in saffron differently depending on the developmental stage. Frontiers in Plant Science, 14, 1107172. https://doi.org/10.3389/fpls.2023.1107172.
Sharma,  K.,  Rok Lee, Y., Park, S. W., & Nile, S. H. (2016). Importance of growth hormones and temperature for physiological regulation of dormancy and sprouting in onions. Food Reviews International, 32 (3), 233–255. https://doi.org//278030918.
Srivastava, N. K., & Srivastava, A. K. (2007). Influence of gibberellic acid on 14CO2 metabolism, growth, and production of alkaloids in Catharanthus roseus. Photosynthetica, 45, 156-160. http://doi.org/10.1007/s11099-007-0026-0.
Tavakoli, Z., Jahani, M., & Hammami, H. (2023). Allelopathic and antifungal effects of saffron (Crocus sativus L.) aqueous extract on germination and seedling growth of London rocket (Sisymbrium irio) and Fusarium solani. Saffron Agronomy & Technology, 11 (3), 321- 341. (In Persian with English abstract). https://doi.org/10.22048/jsat.2023.409228.1499.
Umrao, V. K., Sharma, V., & Kumar, B. (2007). Influence of gibberellic acid spraying on gladiolus cv. Rose Delight. Progressive Agriculture, 7, (1-2), 187-188.
Ziaei, S. M., Feizi, H.,  Khashei Siuki, A.,  & Sahabi, H. (2024). Effect of mother corm priming on physiological characteristics and daughter corms of saffron (Crocus sativus L.) under drought stress conditions. Journal of Saffron Agronomy & Technology, 12 (1), 27-40. (In Persian with English abstract). https://doi.org/10.22048/jsat.2024.436406.1519.