زراعت و فناوری زعفران

زراعت و فناوری زعفران

کاهش استرس اکسیداتیو در دیابت: نقش کروسین و سافرانال

نوع مقاله : مقاله علمی پژوهشی

نویسندگان
محبوبه ناصری 1
علیرضا رامندی 2
راحله رهباریان 3
1 استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی دانشگاه تربت حیدریه، تربت حیدریه، ایران
2 دانشجوی دکتری ابیوتکنولوژی، گروه مهندسی تولید و ژنتیک گیاهی دانشکده کشاورزی دانشگاه فردوسی مشهد، ایران
3 استادیار گروه زیست شناسی، دانشگاه پیام نور
10.22048/jsat.2024.421615.1512
چکیده
دیابت با افزایش استرس اکسیداتیو و کاهش پتانسیل آنتی اکسیدانی به دلیل افزایش تشکیل رادیکال های آزاد همراه است. خاصیت آنتی اکسیدانی کلاله زعفران گزارش شده است. به نظر می‌رسد سطح و عملکرد BDNF و فاکتور رشد عصبی (NGF) در دیابت به دلیل وجود مقاومت به انسولین مختل می‌شود. تغییر سطح BDNF و NGF با افزایش دیابت نوع 2 مرتبط است. این مطالعه با هدف بررسی اثر کروسین و سافرانال بر فعالیت آنزیم های آنتی‌اکسیدانی مانند سوپراکسید دیسموتاز (SOD)، گلوتاتیون پراکسیداز (GPx) و کاتالاز و فاکتور نوروتروفیک مشتق از مغز و عصب (BDNF) انجام شد.در این مطالعه 36 موش صحرایی به 6 گروه به شرح زیر تقسیم شدند: گروه کنترل، دیابتی درمان نشده، دیابتی تحت درمان با دو غلظت 100 و 50 میلی-گرم بر میلی‌لیتر از کروسین و سافرانال به صورت تزریق داخل صفاقی در یک بازه 25 روزه. در پایان دوره درمان، بافت مغز برای ارزیابی آنزیم‌های آنتی‌اکسیدانی BDNF و NGF تشریح شد.سطوح BDNF، NGF، SOD، GPX، CAT و MDA در گروه تیمار با غلظت 100 میلی‌گرم بر میلی‌لیتر کروسین و سافرانال در مقایسه با گروه تحت درمان با غلظت‌50 میلی‌گرم بر میلی‌لیتر کروسین، سافرانال و گروه کنترل افزایش معنی‌داری داشت.نتایج نشان داد که کروسین و سافرانال شاخص‌های آنتی اکسیدانی و آسیب‌های ناشی از دیابت را در بافت مغز موش‌های دیابتی بهبود داده است.
کلیدواژه‌ها
آنتی اکسیدان
مغز
دیابت
مقاومت به انسولین
موش صحرایی نر

موضوعات

عنوان مقاله English

Mitigating Oxidative Stress in Diabetes: The Role of Crocin and Safranal

نویسندگان English

Mahboobeh Naseri 1
Alireza Ramandi 2
Raheleh Rahbarian 3
1 Assistant Professor, Department of Plant Production, Faculty of Agriculture, University of Torbatheydarieh, Torbatheydarieh, Iran.
2 Ph.D. Student of Agrotechnology – Biotechnology, Department of Plant Production and Genetics, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Department of Biology, University of Paymnoor, Iran
چکیده English

Diabetes is linked to heightened oxidative stress and diminished antioxidant potential, stemming from an increased generation of free radicals. The reported antioxidant attributes of saffron crocin highlight its potential in mitigating oxidative stress. The levels and functionality of BDNF (Brain-Derived Neurotrophic Factor) and NGF (Nerve Growth Factor) seem to undergo alterations in diabetes, primarily due to insulin resistance. These fluctuations in BDNF and NGF levels are intricately associated with the progression of type 2 diabetes.This study sought to explore the impact of crocin and safranal on the activity of antioxidant enzymes, including Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Catalase (CAT), and neurotrophic factors derived from the brain and nerve (BDNF). Thirty-six male rats were categorized into six groups: a control group, an untreated diabetic group, and diabetic groups subjected to intraperitoneal injection of two concentrations (100 and 50 milligrams per milliliter) of crocin and safranal over a 25-day duration. At the conclusion of the treatment period, brain tissue dissection was performed to assess antioxidant enzymes BDNF and NGF.In the treatment group with a concentration of 100 milligrams per milliliter of crocin and safranal, there was a notable increase in BDNF, NGF, SOD, GPX, CAT, and MDA levels compared to the group treated with a concentration of 50 milligrams per milliliter of crocin, safranal, as well as the control group. These results suggest that crocin and safranal effectively enhance antioxidant markers and alleviate diabetes-related damages in the brain tissue of diabetic rats

کلیدواژه‌ها English

antioxidant
brain
diabetes
insulin resistance
Male rat
Ansari, S., Djalali, M., MohammadzadehHonarvar, N., Mazaherioun,M., Zarei, M., & Gholampour, Z. (2016). Assessing the effect of omega-3 fatty acids supplementation on serumBDNF (brain derived neurotrophic factor) in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled study. International Research Journal of Biological Sciences, 10 (4), 380-383. https://doi.org/10.1159/000321804.
Eslami, R., Sorkhkamanzadeh, G., Kazemi, A.,Gharakhanlou, R., & Banaifar, A. (2015). Effect of 6-week endurance training on bdnf expression in motor root of spinal cord in rats with diabetic neuropathy. Mazandaran. Journal of Shahrekord University of Medical Sciences, 25 (124), 94-110. https://doi.org/10.22038/JNFH.2019.37231.1163.
Ghojagh, D., DeylamKatoli, H., & HabibiNodeh, M. (2013). Level of glutathione peroxidase activity and carbonyl and malondialdelhyde levels in eryth-rocyte of diabetic rats. Human Movement Science, 9 (3), 179-83. https://doi.org/10.1016/j.cccn.2003.09.023.
Handsaker, J. C., Brown, S. J., Bowling, F. L., Maganaris, C. N., Boulton, A. J. M., & Reeves, N. D. (2016). Resistance exercise training increases lower limb speed of strength generation during stair ascent and descent in people with diabetic peripheral neuropathy. Diabetic Medicine, 33 (1), 97-104. https://doi.org/10.1111/dme.12841.
Hosseinzadeh, H., & Nassiri-Asl, M. (2013). Avicenna̕ s (IbnSina) the canon of medicine and saffron (Crocus sativus): a review. Phytotherapy Research, 27, 475–483.  https://doi.org/10.1002/ptr.4784.
Kianbakht, S., & Hajiaghaee, R. (2011). Anti-hyperglycemic effects of saffron and its active constituents, crocin and safranal, in alloxan-induced dia-beticrats. Journal of Medicinal Plants, 3 (39), 82-90. https://doi.org/10.1016/0378-8741(95)01279-M.
Maritim, A.C., Sanders, R.A., & Watkins, J. B. (2003). Effects of α-lipoic acid on biomarkers of oxidative stress in streptozotocin-induced diabetic rats. Journal of Nutritional Biochemistry, 14 (5), 288-294. https://doi.org/10.1016/S0955-2863(03)00036-6.
Naseri, M., Ramandi, A., & Mohammaditazeabadi, F. (2023). Effect of saffron corm aqueous extract on blood glucose level enzymes of liver tissue in Streptozotocin-induced diabetic rats. Saffron Agronomy and Technology, 11 (1), 87-97. ( In Persian with English Summary). https://doi.org/10.22048/jsat.2023.365486.1471.
Nazari, H., Heydarpoor, S., MohamadiMofrad, A., Nazari, Y., & Nazari, A. (2016). Effect of vitamin C on serum concentration of brain-derived neurotrophic factor among healthy inactive young men. Shefaye Khatam, 4 (2), 27-32. https://doi.org/10.22048/jsat.2023.365486.1471.
Rahbarian, R., SepehriMoghadam, H., & Sadoughi, S. D. (2016). Effect of aqueous extract of Launaea acanthodes on open skin wound in diabetic rats. Human Movement Science, 22 (1), 1-11. https://doi.org/10.18869/acadpub.hms.22.1.1.
Rai, O., Mishra, V., Chandra, R., Saxena, S., & Mangal, B. (2016). Diabetic peripheral neuropathy and its metabolic determinants in a north Indian population. National Journal of Integrated Research in Medicine, 7 (2), 1-4. https://doi.org/10.22048/jsat.2023.365486.1471.
Ramandi, A., Gholizadegan, A., & Seifi, A. (2022a). Optimization of callogenesis and cell suspension culture in saffron. Journal of Saffron Research, 11 (1). (In Persian with English Summary). https://doi.org/10.22077/jsr.2022.5718.1198.
Ramandi, A., Javan, I. Y., Tazehabadi, F. M., veAsl, G. I., Khosravanian, R., & Ebrahimzadeh, M. H. (2019). Improvement in seed surface sterilization and in vitro seed germination of ornamental and medicinal plant-Catharanthus roseus (L.). Chiang Mai Journal of Science, 46 (6), 1107–1112. https://doi.org/10.1007/s11274-022-03450-x.
Ramandi, A., Nourashrafeddin, S. M., Marashi, S. H., & Seifi, A. (2023a). Microbiome contributes to phenotypic plasticity in saffron crocus. World Journal of Microbiology and Biotechnology, 39 (1), 1-13. https://doi.org/10.1007/s11274-022-03450-x.
Ramandi, A., Naseri, M., & Yousefijavan, I. (2022c). The effect of aqueous extract of Crocus sativus style on blood coagulation Indices in rats. Journal of Saffron Research, 10 (1), 168-160.‏ ( In Persian with English Summary). https://doi.org/10.22077/jsr.2022.4950.1176.
Rahbarian, R., SepehriMoghadam, H., & Sadoughi, S. D. (2015). Effect of aqueous extract of Launaea acanthodes on testicular tissue and sperm parameters in alloxan-induced diabetic rats (Persian). Human Movement Science, 21 (1), 9-21. https://doi.org/10.18869/acadpub.hms.21.1.21.
Samarghandian, S., Asadi-Samani, M., Farkhondeh, T., & Bahmani, M. (2016). Assessment the effect of saffron ethanolic extract (Crocus sativus L.) on oxidative damages in aged male rat liver. Der Pharmacia Lettre, 8 (3), 283-290.‏ https://doi.org/10.1016/j.phymed.2006.11.028.
Shirazi, A., Golab, F., Sanadgol, N., Barati, M., Mohammad Salehi, R., & Vahabzadeh, G. (2016). Evaluationof the neurotrophic factors in animal model of myelin destruction induced by cuprizone in c57bl/6 mice. Shefaye Khatam, 4 (2), 47-54. https://doi.org/10.2337/diacare.27.5.1047.
Tamaddonfard, E., Farshid, A. A., Maroufi, S., Kazemi-Shojaei, S., Erfanparst, A., Asri-Rezaei, S., Taati, M., Dabbaghi, M., & Escort. M. (2014). Effects of safranal, a constituent of saffron, and vitamin E on nerve functions and histopathology following crush injury of sciatic nerve in rats. Phytomedicine, 21, 717–723. https://doi.org/10.1016/j.phymed.2013.10.031.
Wild, S., Roglic, G., Green, A., Sicree, R., & King, H. (2004).  Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care, 27, 1047–1053. https://doi.org/10.2337/diacare.27.5.1047.
Xi, L., Qian, Z., Du, P., & Fu, J. Pharmacokinetic properties of crocin (crocetin digentiobiose ester)  following oral administration in rats.  Phytomedicine, 4 (9), 633–666. https://doi.org/10.1016/j.phymed.2006.11.028.